果蝇NAD+依赖性乙醛氧化

Francoise Garcin, Denis Kasiencsuk, Simone Radouco-Thomas, Johanne Cote, Corneille Radouco-Thomas
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引用次数: 6

摘要

1.1. 用分光光度法测定黑腹果蝇匀浆中NAD+依赖性乙醛氧化。该反应是由乙醛的加入引起的,并在5分钟内线性进行。在低乙醛浓度下,反应速率最快,而较高的乙醛浓度则被证明是抑制的。NAD+浓度对酶活性的影响遵循经典的米切里斯动力学。NAD+的视Km为0.05 mM.5.5。这些数据为NAD+依赖性醛脱氢酶的存在提供了证据。这表明这种酶参与果蝇乙醛的氧化。6.6。讨论了该酶在乙醇代谢和乙醇耐受性方面的生物化学和生物学意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
NAD+-dependent acetaldehyde oxidation in Drosophila

  • 1.

    1. NAD+-dependent acetaldehyde oxidation was measured spectrophotometrically in homogenates of flies Drosophila melanogaster.

  • 2.

    2. The reaction was specifically triggered by addition of acetaldehyde and proceeded linearily over five minutes.

  • 3.

    3. At low acetaldehyde concentrations the reaction rate was rapidly maximal whereas higher acetaldehyde concentrations proved to be inhibitary.

  • 4.

    4. Enzyme activity in regard to NAD+ concentration followed classical Michaelis kinetics. The apparent Km for NAD+ was 0.05 mM.

  • 5.

    5. These data provide evidence for the presence of a NAD+-dependent aldehyde dehydrogenase. It is suggested that this enzyme is involved in the oxidation of acetaldehyde in Drosophila.

  • 6.

    6. The biochemical and biological significance of this enzyme in regard to ethanol metabolism and ethanol tolerance is discussed.

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