Robert K. Montgomery, Mariana A. Sybicki, Ann G. Forcier, Richard J. Grand
{"title":"大鼠肠微绒毛膜蔗糖-异麦芽糖酶是一种单一的高分子量蛋白，在无腔内因子的情况下具有充分的活性","authors":"Robert K. Montgomery, Mariana A. Sybicki, Ann G. Forcier, Richard J. Grand","doi":"10.1016/0005-2744(81)90024-3","DOIUrl":null,"url":null,"abstract":"<div><p>Sucrase-isomaltase immunoprecipitated from brush border of an intestinal transplant lacking pancreatic proteases was found to be a single, high molecular weight protein. Elastase digestion converted this protein into two subunits which co-migrated on electrophoresis with those normally found on the microvillus membrane. The high molecular weight from had full sucrase and isomaltase activities.</p></div>","PeriodicalId":100159,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology","volume":"661 2","pages":"Pages 346-349"},"PeriodicalIF":0.0000,"publicationDate":"1981-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2744(81)90024-3","citationCount":"32","resultStr":"{\"title\":\"Rat intestinal microvillus membrane sucrase-isomaltase is a single high molecular weight protein and fully active enzyme in the absence of luminal factors\",\"authors\":\"Robert K. Montgomery, Mariana A. Sybicki, Ann G. Forcier, Richard J. Grand\",\"doi\":\"10.1016/0005-2744(81)90024-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Sucrase-isomaltase immunoprecipitated from brush border of an intestinal transplant lacking pancreatic proteases was found to be a single, high molecular weight protein. Elastase digestion converted this protein into two subunits which co-migrated on electrophoresis with those normally found on the microvillus membrane. The high molecular weight from had full sucrase and isomaltase activities.</p></div>\",\"PeriodicalId\":100159,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Enzymology\",\"volume\":\"661 2\",\"pages\":\"Pages 346-349\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-10-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0005-2744(81)90024-3\",\"citationCount\":\"32\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Enzymology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0005274481900243\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005274481900243","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Rat intestinal microvillus membrane sucrase-isomaltase is a single high molecular weight protein and fully active enzyme in the absence of luminal factors
Sucrase-isomaltase immunoprecipitated from brush border of an intestinal transplant lacking pancreatic proteases was found to be a single, high molecular weight protein. Elastase digestion converted this protein into two subunits which co-migrated on electrophoresis with those normally found on the microvillus membrane. The high molecular weight from had full sucrase and isomaltase activities.
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