布鲁氏锥虫磷脂酶A1的分离。

Tropenmedizin und Parasitologie Pub Date : 1984-03-01

P N Hambrey, C M Forsberg, A Mellors, I R Tizard, G M Werchola

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引用次数: 0

摘要

采用磷脂酰胆碱- sepharose亲和柱层析、deae -纤维素阴离子交换层析和Sephacryl S-200分子排斥层析，对布氏锥虫的磷脂酶A1进行了380倍的纯化。辛基琼脂糖疏水柱层析可以代替PC-Sepharose柱。sds -聚丙烯酰胺凝胶电泳发现，锥虫PLase A1的分子量为26000。

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Isolation of phospholipase A1 from Trypanosoma brucei.

Phospholipase A1 from Trypanosoma brucei brucei has been purified 380-fold by column chromatography on phosphatidylcholine-Sepharose affinity columns followed by DEAE-cellulose anion-exchange chromatography and Sephacryl S-200 molecular exclusion chromatography. Octyl-Agarose hydrophobic column chromatography can be substituted for the PC-Sepharose column. The molecular weight of trypanosomal PLase A1 was found to be 26,000 by SDS-polyacrylamide gel electrophoresis.

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Tropenmedizin und Parasitologie

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