[体外培养7日龄鸡胚皮肤愈合]。

A Thevenet
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引用次数: 0

摘要

采用光镜、透射电镜和扫描电镜对7日龄鸡胚背胸腰椎皮肤进行了体外创伤愈合形态学研究。一个直径750微米的圆形伤口被从背部皮肤上打出来,剥去了表皮和下面致密的真皮层。伤口愈合在96 ~ 120小时内完成。伤口部位未见羽芽发育。伤后约24 h,表皮开始迁移;表皮细胞的迁移比基底表皮细胞的迁移早约12小时。表皮迁移的机制与5天龄的鸡胚在伤口愈合过程中观察到的相似(THEVENET, 1981),在损伤后12小时,裸露真皮的浅表上皮就发生了。真皮细胞的细胞质中有许多微管,粗糙的内质网扩张。在最初的48小时内,表皮细胞与上皮真皮细胞突起建立了直接接触和紧密平行的并列区。创面边缘基底膜层致密,无回缩或皱褶。迁移的表皮细胞和上皮真皮细胞的细胞质呈现出许多细胞骨架结构。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Cicatrization of the skin of the 7-day-old chick embryo cultured in vitro].

A morphological study of in vitro wound healing has been performed by light, transmission and scanning electron microscopy in dorsal thoraco-lumbar skin of 7-day chick embryos. A circular wound, 750 microns in diameter, was punched out of dorsal skin, removing epidermis and the underlying dense dermis. Wound closure was completed within 96 to 120 hours. Feather bud development was not observed at the wound site. The epidermis began to migrate some 24 h after the wounding; the migration of peridermal cells preceded that of basal epidermal cells by some 12 hours. Mechanisms of the epidermal migration were similar to those observed in situ during wound healing of the integument in 5-day chick embryos (THEVENET, 1981), Superficial epithelization of bare dermis occurred as soon as 12 h after the injury. Cytoplasm of dermal cells exhibited many microtubules and a dilated rough endoplasmic reticulum. During the first 48 h, the epidermal cells established direct contacts and zones of close parallel apposition with epithelized dermal cell processes. The basement membrane lamina densa was maintained at the edges of the wound without retraction or ruffling. It was reconstituted concomitantly with the epidermal migration within 72 h. Cytoplasm of migratory epidermal and epithelized dermal cells exhibited many cytoskeleton structures.

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