荧光光谱法测定人血小板。

Biorheology. Supplement : the official journal of the International Society of Biorheology Pub Date : 1984-01-01 DOI:10.3233/bir-1984-23s158

M L Viriot, T Collin, S Gaillard, J F Stoltz, J C Andre

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引用次数: 0

摘要

研究了人血小板中二苯基丙烷分子内准分子的形成。准分子与单体的发射强度之比(IE/IM)对脂质膜结构的变化很敏感，这种变化是由外部扰动引起的，如半琥珀酸胆固醇的引入和温度。缺乏可靠的“微粘度”测定可能是由于探针在水细胞悬浮液中的溶解度差。结果与荧光偏振法进行了比较。

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Investigation of human blood platelets with fluorescence spectroscopy.

Intramolecular excimer formation of dipyrenylpropane was investigated in human blood platelets. The ratio of the emission intensities of excimer to monomer (IE/IM) is sensitive to changes in lipidic membrane structure, induced by external perturbations, such as cholesteryl hemisuccinate introduction and temperature. Lack of reliable "microviscosities" determinations could be due to poor solubility of the probe in aqueous cells suspensions. Results were compared to those obtained with the fluorescence polarization technique.

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Biorheology. Supplement : the official journal of the International Society of Biorheology

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