仓鼠颊袋上皮细胞间细胞间隙体积的改变:正常和致癌处理组织的定量研究。

F H White, K Gohari
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引用次数: 30

摘要

本报告通过量化仓鼠颊袋癌变过程中细胞间隙的体积,在超微结构水平上研究了被称为“细胞粘附性丧失”的上皮发育不良特征的程度。局部应用DMBA后,病变分为增生、不典型增生和癌,未治疗的眼袋作为对照。使用立体点计数程序来确定每组在确定的基层、棘层和颗粒层中的细胞间空间的体积密度。一般来说,在癌变过程中,每一层的体积密度都在逐渐增加。这些结果表明,在癌变过程中上皮细胞的分离增加,尽管尚不清楚这是由于特化(即桥粒体)或非特化膜区域之间的内聚丧失造成的。此外,病理改变的一个简单指标,病理改变比率(PAR),被描述并用于评估现有的各种口腔粘膜条件下细胞间隙的已发表数据。研究发现,在致癌物处理的上皮中,PAR值明显高于描述伤口愈合、扁平苔藓和单纯白斑变化的报告。这些客观的技术对研究病变上皮的发病机制有价值,并可能在口腔癌前病变的诊断中得到应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alterations in the volume of the intercellular space between epithelial cells of the hamster cheek-pouch: quantitative studies of normal and carcinogen-treated tissues.

The present report investigated the extent of the epithelial dysplastic feature known as "loss of cellular adherence" at the ultrastructural level by quantifying the volume of the intercellular space during hamster cheek-pouch carcinogenesis. Following topical application of DMBA to cheek-pouches, lesions were classified as hyperplasia, dysplasia and carcinoma, with untreated pouches serving as a control. Stereological point counting procedures were used to determine the volume density of intercellular space in defined basal, spinous and granular layers for each group. In general, progressive increases in volume density were detected within each stratum during carcinogenesis. These results indicate that increasing separation of epithelial cells occurs during carcinogenesis, although it is not yet known whether this results from loss of cohesion between specialised (i.e., desmosomal) or nonspecialised membrane areas. In addition, a simple indicator of pathological alteration, the Pathological Alteration Ratio (PAR), is described and was used to evaluate existing published data for intercellular spaces in various oral mucosal conditions. Values of the PAR were found to be substantially higher in carcinogen-treated epithelia than in reports describing changes in wound healing, lichen planus and leukoplakia simplex. These objective techniques are of value for investigating the pathogenesis of diseased epithelium and may find applications in the diagnosis of oral premalignant lesions.

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