[胰腺B细胞高尔基体胰岛素抗原位点的膜结合]。

L Orci, M Ravazzola, A Perrelet
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引用次数: 0

摘要

使用抗胰岛素抗血清的超微结构免疫细胞化学可以检测到位于胰腺b细胞高尔基体池内侧的胰岛素抗原位点。相比之下,在高尔基体释放的分泌颗粒中,胰岛素抗原位点定位在颗粒内容物上,而不是在膜上。高尔基膜水平上的胰岛素抗原位点与特定胰岛素受体的存在和/或胰岛素与膜之间的电荷相互作用是相容的。在这个水平上的结合可能反过来参与胰岛素多肽的分选,注定要集中在分泌颗粒中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Membrane binding of insulin antigenic sites in the Golgi apparatus of pancreatic B cells].

Ultrastructural immunocytochemistry with anti-insulin antiserum allowed the detection of insulin antigenic sites localized at the inner aspect of the cisternae of the Golgi apparatus in pancreatic B-cells. By contrast, in secretory granules released from the Golgi apparatus, insulin antigenic sites were localized over the granule content but not on the membrane. The demonstration of insulin antigenic sites at the level of the Golgi membranes is compatible with the presence of specific insulin receptors and/or charge interactions between the insulin and the membrane. Binding at this level could in turn be involved in the sorting out of insulin polypeptides destined to be concentrated in secretory granules.

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