[异硫氰酸荧光素(FITC)和辣根过氧化物酶(HPOD)抗体的双重标记]。

Microscopica acta Pub Date : 1983-01-01
U Warweg
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引用次数: 0

摘要

本文提出了一种用异硫氰酸荧光素(FITC)和辣根过氧化物酶(HPOD)标记抗体的方法。利用这种双标记抗血清,对同一对象逐点进行荧光-血清学抗体技术(FAT)和酶-血清学抗体技术(EAT)。所展示的图片如何展示两种技术的结果,FAT和EAT,与所描述的方法是相同的。因此,通过对HPOD的后续组织化学证明,在荧光显微镜下开发后,有可能获得具有相同生物学结果的永久制剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Double labeling of antibodies with fluorescein isothiocyanate (FITC) and horseradish peroxidase (HPOD)].

This paper presents a method for labeling antibodies with fluorescein isothiocyanate (FITC) and additional with horseradish peroxidase (HPOD). With this double labelled antisera the fluorescence-serologic antibody technique (FAT) as well as the enzyme-serologic antibody technique (EAT) was done bit by bit for the same object. How the presented pictures demonstrate the result of both techniques, FAT and EAT, are the same with the described method. So, it is possible after fluorescence-microscopical exploitation through a following histochemical proof of the HPOD to get permanent preparations with the same biological result.

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