以滋养体为抗原的免疫荧光法检测肠第鞭毛虫血清抗体。

Tropenmedizin und Parasitologie Pub Date : 1984-03-01
J Winiecka, W Kasprzak, W Kociecka, J Płotkowiak, P Myjak
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引用次数: 0

摘要

采用间接免疫荧光法检测88例有症状和无症状贾第鞭毛虫病患者血清中的抗贾第鞭毛虫抗体,并以无菌培养的肠贾第鞭毛虫滋养体为抗原。以40名健康受试者的血清作为对照组,并对28名其他肠道寄生虫患者(14名患有肌囊虫,14名患有有症状的溶组织内阿米巴感染)的血清进行了调查,以确定测试的特异性。贾第虫病患者血清中有82%的患者血清中存在1∶16 ~ 1∶256的抗体。66%的贾第虫病患者滴度至少为1:32,可作为诊断和指示性感染。18例急性贾第虫病患者滴度至少为1:32。来自健康对照者或感染其他肠道寄生虫的患者的血清中,只有5份(7%)在1:大于或等于32的稀释液中有反应。间接免疫荧光法可用于检测血清中肠弧菌的抗体。然而,在慢性感染者中经常出现阴性结果,这表明需要对贾第虫病的血清学检测进行更严格的评估。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serum antibodies to Giardia intestinalis detected by immunofluorescence using trophozoites as antigen.

Anti-Giardia antibodies were studied in 88 sera of patients with symptomatic and asymptomatic giardiasis by using the indirect immunofluorescent test and Giardia intestinalis trophozoites from an axenic culture as antigen. The sera of 40 healthy subjects were used as a control group and of 28 patients with other intestinal parasites (14 with Sarcocystis sp. and 14 with symptomatic Entamoeba histolytica infection) have been investigated for determination of test specificity. 82% of sera from patients with giardiasis presented G. intestinalis antibodies at titers of 1:16 to 1:256. Titers of at least 1:32, accepted as diagnostic and indicative of the infection, were found in 66% of patients with giardiasis. All of 18 patients with acute course of giardiasis had titers of at least 1:32. Only 5 sera (7%) from healthy control subjects or from patients infected with other intestinal parasites reacted at dilutions of 1: greater than or equal to 32. The indirect immunofluorescence test proves to be useful in detecting antibodies to G. intestinalis in the serum. However, the negative results occurring rather frequently in persons with chronic infection indicate a need for a more critical evaluation of serological tests in giardiasis.

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