Multiparameter flow cytometry. Part II. application in hematology.

The application of flow cytometry to hematology is emerging as an important new technique in experimental and clinical leukemic research. In addition to the described methods to determine DNA, RNA, and esterase content and the stability of DNA in situ, quantitation of immunofluorescence using antibody techniques has greatly elucidated the hematopoietic cell system. Hence, flow cytometry provides new perspective for determining the origin, diagnosis, classification, staging and treatment scheduling of normal hematopoietic and malignant cell populations.