胰蛋白酶诱导胸腺原细胞的分化。

E H Eylar, H H Fudenberg
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引用次数: 0

摘要

较低浓度(1 ~ 50微克/毫升)胰蛋白酶体外处理裸鼠脾胸腺原细胞可诱导th1 -向th1 +转化。事先加热或添加大豆胰蛋白酶抑制剂可抑制胰蛋白酶的作用。胰蛋白酶处理的最大效果是,约25%的脾脏细胞从白蛋白梯度的B层转化为β阳性细胞,与胸腺激素制剂(TP-1)在10 ng/ml时的效果相当。最大转化需要与TP-1或胰蛋白酶孵育120分钟。我们得出结论,胰蛋白酶和TP-1都是通过干扰膜受体来起作用的,这可以发送信号启动分化过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differentiation of prothymocytes induced by trypsin.

Treatment in vitro of nude mouse spleen prothymocytes with relatively low concentrations (1-50 micrograms/ml) of trypsin induce the Thy 1- to Thy 1+ conversion. The effect of trypsin was inhibited by prior heating or by addition of soybean trypsin inhibitor. The maximal effect achieved by trypsin treatment, approximately 25% conversion of spleen cells from layer B of an albumin gradient into theta-positive cells, was equivalent to that obtained with thymic hormone preparation (TP-1) at 10 ng/ml. Maximal conversion required 120 min incubation with TP-1 or trypsin. We conclude that both trypsin and TP-1 act by perturbation of a membrane receptor which can send a signal initiating the differentiation process.

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