金鱼肝微粒体中药物氧化单加氧酶系统。

Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology Pub Date : 1983-01-01

S Maemura, T Omura

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引用次数: 0

摘要

研究了鲫鱼肝脏的分离方法，并对微粒体部分的性质进行了检测。微粒体片段含有细胞色素P-450，并催化氨基吡啶、苯胺、7-乙氧基香豆素和苯并(a)芘的氧化。其氧化活性明显低于大鼠肝微粒体。用氰化钾滴定细胞色素P-450表明金鱼肝微粒体中存在多种形式的细胞色素P-450。用含3-甲基胆蒽的食物喂养金鱼，极大地诱导了肝微粒体苯并(a)芘羟基化活性。细胞色素P-450一氧化碳化合物的Soret峰由450 nm移至448 nm。

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Drug-oxidizing mono-oxygenase system in liver microsomes of goldfish (Carassius auratus).

The fractionation of the liver of goldfish (Carassius auratus) was studied, and the properties of the microsomal fraction were examined. The microsomal fraction contained cytochrome P-450 and catalyzed the oxidation of aminopyrine, aniline, 7-ethoxycoumarin and benzo(a)pyrene. The oxidation activities were significantly lower than those of rat liver microsomes. The titration of cytochrome P-450 by potassium cyanide indicated the presence of multiple forms of cytochrome P-450 in goldfish liver microsomes. Feeding of goldfish with 3-methylcholanthrene-containing food greatly induced benzo(a)pyrene hydroxylation activity of the liver microsomes. The Soret peak of the carbon monoxide compound of cytochrome P-450 was shifted from 450 to 448 nm.

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Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology

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