一种新的成人t细胞白血病病毒血清抗体凝集试验。

Gan Pub Date : 1984-10-01

M Ikeda, R Fujino, T Matsui, T Yoshida, H Komoda, J Imai

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引用次数: 0

摘要

建立了一种新的明胶颗粒凝集试验，用于测定成人t细胞白血病病毒(ATLV/HTLV-I)天然抗体。从病毒产生细胞株培养液中提取部分纯化的病毒抗原，包被在人工明胶颗粒载体上。凝集试验测定的抗体滴度与间接免疫荧光法测定的抗体滴度高度相关。凝集试验简单、灵敏、特异，可用于人类血清病毒抗体的大规模筛选。

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A new agglutination test for serum antibodies to adult T-cell leukemia virus.

A new gelatin particle agglutination test was developed for assay of natural antibodies to adult T-cell leukemia virus (ATLV/HTLV-I). Partially purified viral antigen from culture fluid of a virus-producer cell line was coated on artificial gelatin particle carriers. A high correlation was observed between the titers of antibodies determined by the agglutination test and by indirect immunofluorescence assay. The agglutination test is simple, sensitive and specific, and should be useful for mass screening of human sera for viral antibodies.

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Gan

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