{"title":"离体组织中葡萄糖和阳离子跨细胞膜转运的关系[j]。无K+培养基、乌沙因和胰岛素对大鼠膈肌葡萄糖命运的影响","authors":"Torben Clausen","doi":"10.1016/0926-6585(66)90303-7","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Incubation of isolated rat hemidiaphragm in a K<sup>+</sup>-free bicarbonate buffer has been shown to induce stimulation of glycogen synthesis and inhibition of lactate production, without change in glucose uptake.</p></span></li><li><span>2.</span><span><p>2. Essentially similar changes were produced when ouabain was added to bicarbonate buffer with a normal K<sup>+</sup> content.</p></span></li><li><span>3.</span><span><p>3. These metabolic changes were therefore considered as secondary to a decrease in active cation transport.</p></span></li><li><span>4.</span><span><p>4. Insulin (0.1 I.U./ml) was shown to stimulate glycogen synthesis, lactate production and glucose uptake.</p></span></li><li><span>5.</span><span><p>5. In the presence of insulin (0.1 I.U./ml), the addition of ouabain gave a further stimulation of glycogen synthesis. This was accompanied by a decrease in lactate production and a slight depression of the glucose uptake.</p></span></li><li><span>6.</span><span><p>6. Furthermore, it was shown that ouabain in concentrations down to 1.4·10<sup>−6</sup> M induced an inhibition of CO<sub>2</sub> production from glucose.</p></span></li></ul></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 3","pages":"Pages 361-368"},"PeriodicalIF":0.0000,"publicationDate":"1966-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90303-7","citationCount":"75","resultStr":"{\"title\":\"The relationship between the transport of glucose and cations across cell membranes in isolated tissues II. Effects of K+-free medium, ouabain and insulin upon the fate of glucose in rat diaphragm\",\"authors\":\"Torben Clausen\",\"doi\":\"10.1016/0926-6585(66)90303-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Incubation of isolated rat hemidiaphragm in a K<sup>+</sup>-free bicarbonate buffer has been shown to induce stimulation of glycogen synthesis and inhibition of lactate production, without change in glucose uptake.</p></span></li><li><span>2.</span><span><p>2. Essentially similar changes were produced when ouabain was added to bicarbonate buffer with a normal K<sup>+</sup> content.</p></span></li><li><span>3.</span><span><p>3. These metabolic changes were therefore considered as secondary to a decrease in active cation transport.</p></span></li><li><span>4.</span><span><p>4. Insulin (0.1 I.U./ml) was shown to stimulate glycogen synthesis, lactate production and glucose uptake.</p></span></li><li><span>5.</span><span><p>5. In the presence of insulin (0.1 I.U./ml), the addition of ouabain gave a further stimulation of glycogen synthesis. This was accompanied by a decrease in lactate production and a slight depression of the glucose uptake.</p></span></li><li><span>6.</span><span><p>6. Furthermore, it was shown that ouabain in concentrations down to 1.4·10<sup>−6</sup> M induced an inhibition of CO<sub>2</sub> production from glucose.</p></span></li></ul></div>\",\"PeriodicalId\":100158,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis\",\"volume\":\"120 3\",\"pages\":\"Pages 361-368\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1966-07-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6585(66)90303-7\",\"citationCount\":\"75\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926658566903037\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926658566903037","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 75
摘要
1.1. 在无K+的碳酸氢盐缓冲液中培养分离的大鼠半膈肌已被证明可以诱导糖原合成的刺激和乳酸生成的抑制，而葡萄糖的摄取没有改变。当将瓦巴因加入到具有正常K+含量的碳酸氢盐缓冲液中时，基本上也会产生类似的变化。因此，这些代谢变化被认为是活性阳离子运输减少的继发性变化。胰岛素(0.1 iu /ml)可刺激糖原合成、乳酸生成和葡萄糖摄取。在胰岛素(0.1 iu /ml)存在的情况下，加入瓦巴因进一步刺激糖原合成。这伴随着乳酸生成的减少和葡萄糖摄取的轻微抑制。此外，研究表明，1.4·10−6 M浓度的瓦苦因对葡萄糖的CO2生成有抑制作用。
The relationship between the transport of glucose and cations across cell membranes in isolated tissues II. Effects of K+-free medium, ouabain and insulin upon the fate of glucose in rat diaphragm
1.
1. Incubation of isolated rat hemidiaphragm in a K+-free bicarbonate buffer has been shown to induce stimulation of glycogen synthesis and inhibition of lactate production, without change in glucose uptake.
2.
2. Essentially similar changes were produced when ouabain was added to bicarbonate buffer with a normal K+ content.
3.
3. These metabolic changes were therefore considered as secondary to a decrease in active cation transport.
4.
4. Insulin (0.1 I.U./ml) was shown to stimulate glycogen synthesis, lactate production and glucose uptake.
5.
5. In the presence of insulin (0.1 I.U./ml), the addition of ouabain gave a further stimulation of glycogen synthesis. This was accompanied by a decrease in lactate production and a slight depression of the glucose uptake.
6.
6. Furthermore, it was shown that ouabain in concentrations down to 1.4·10−6 M induced an inhibition of CO2 production from glucose.
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