{"title":"硬骨鱼肝脏中天冬氨酸产氨的机制","authors":"Ralph Janicki , Jo Lingis","doi":"10.1016/0010-406X(70)90962-X","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Ammonia production from <span>l</span>-aspartate was studied in liver homogenates from four species of teleosts: <em>Myoxocephalus scorpius, M octodecimspinosus, Pseudopleuronectes americanus</em> and <em>Anguilla rostrata</em>. Liver homogenates from two species of elasmobranchs, <em>Squalus acanthias</em> and <em>Raja erinacea</em>, failed to liberate ammonia from <span>l</span>-aspartate.</p></span></li><li><span>2.</span><span><p>2. No ammonia was liberated from <span>l</span>-aspartate in liver homogenates from <em>M. scorpius</em> in the presence of transaminase inhibitors: 4 × 10<sup>−3</sup> M semicarbizide, 5 × 10<sup>−3</sup> M isonicotinoyl hydrazide and 4 × 10<sup>−3</sup> M hydroxylamine.</p></span></li><li><span>3.</span><span><p>3. Single subcellular fractions, nuclear, mitochondrial and cytoplasmic, failed to produce ammonia from <span>l</span>-aspartate. However, when mitochondrial and cytoplasmic fractions were combined, ammonia was produced from <span>l</span>-aspartate.</p></span></li><li><span>4.</span><span><p>4. The results indicated a transamination reaction is involved in the liberation of ammonia from <span>l</span>-aspartate in teleost liver.</p></span></li></ul></div>","PeriodicalId":78189,"journal":{"name":"Comparative biochemistry and physiology","volume":"37 1","pages":"Pages 101-105"},"PeriodicalIF":0.0000,"publicationDate":"1970-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0010-406X(70)90962-X","citationCount":"11","resultStr":"{\"title\":\"Mechanism of ammonia production from aspartate in teleost liver\",\"authors\":\"Ralph Janicki , Jo Lingis\",\"doi\":\"10.1016/0010-406X(70)90962-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Ammonia production from <span>l</span>-aspartate was studied in liver homogenates from four species of teleosts: <em>Myoxocephalus scorpius, M octodecimspinosus, Pseudopleuronectes americanus</em> and <em>Anguilla rostrata</em>. Liver homogenates from two species of elasmobranchs, <em>Squalus acanthias</em> and <em>Raja erinacea</em>, failed to liberate ammonia from <span>l</span>-aspartate.</p></span></li><li><span>2.</span><span><p>2. No ammonia was liberated from <span>l</span>-aspartate in liver homogenates from <em>M. scorpius</em> in the presence of transaminase inhibitors: 4 × 10<sup>−3</sup> M semicarbizide, 5 × 10<sup>−3</sup> M isonicotinoyl hydrazide and 4 × 10<sup>−3</sup> M hydroxylamine.</p></span></li><li><span>3.</span><span><p>3. Single subcellular fractions, nuclear, mitochondrial and cytoplasmic, failed to produce ammonia from <span>l</span>-aspartate. However, when mitochondrial and cytoplasmic fractions were combined, ammonia was produced from <span>l</span>-aspartate.</p></span></li><li><span>4.</span><span><p>4. The results indicated a transamination reaction is involved in the liberation of ammonia from <span>l</span>-aspartate in teleost liver.</p></span></li></ul></div>\",\"PeriodicalId\":78189,\"journal\":{\"name\":\"Comparative biochemistry and physiology\",\"volume\":\"37 1\",\"pages\":\"Pages 101-105\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1970-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0010-406X(70)90962-X\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative biochemistry and physiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0010406X7090962X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative biochemistry and physiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0010406X7090962X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Mechanism of ammonia production from aspartate in teleost liver
1.
1. Ammonia production from l-aspartate was studied in liver homogenates from four species of teleosts: Myoxocephalus scorpius, M octodecimspinosus, Pseudopleuronectes americanus and Anguilla rostrata. Liver homogenates from two species of elasmobranchs, Squalus acanthias and Raja erinacea, failed to liberate ammonia from l-aspartate.
2.
2. No ammonia was liberated from l-aspartate in liver homogenates from M. scorpius in the presence of transaminase inhibitors: 4 × 10−3 M semicarbizide, 5 × 10−3 M isonicotinoyl hydrazide and 4 × 10−3 M hydroxylamine.
3.
3. Single subcellular fractions, nuclear, mitochondrial and cytoplasmic, failed to produce ammonia from l-aspartate. However, when mitochondrial and cytoplasmic fractions were combined, ammonia was produced from l-aspartate.
4.
4. The results indicated a transamination reaction is involved in the liberation of ammonia from l-aspartate in teleost liver.
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