{"title":"介绍了分离DNA的色谱方法","authors":"R.M. Kothari","doi":"10.1016/0009-5907(70)80008-4","DOIUrl":null,"url":null,"abstract":"<div><p>Ion-exchange resins and inorganic gels have only limited application in the fractionation of DNA. Although DEAE-cellulose has been used extensively, ECTEO-LA-cellulose has promise in the fractionation of DNA. Carboxymethylcellulose or nitrocellulose have limited capabilities as regards DNA fractionation. Gel filtration techniques employing agar gels, DEAE-Sephadex and DEAE-dextran are of more value in the fractionation of s-RNA and polynucleotides than for highly polymerised DNA. Amongst the kieselguhr columns, the methylated serum albumin—kieselguhr column found wide applicability and gives reproducible profiles. An Amberlite IR-120 Al<sup>3+</sup> column also gives interesting results with various potential applications. Alternate methods effecting finer resolution are still a pressing need.</p></div>","PeriodicalId":78892,"journal":{"name":"Chromatographic reviews","volume":"12 2","pages":"Pages 127-155"},"PeriodicalIF":0.0000,"publicationDate":"1970-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0009-5907(70)80008-4","citationCount":"15","resultStr":"{\"title\":\"Present chromatographic methods of fractionating DNA\",\"authors\":\"R.M. Kothari\",\"doi\":\"10.1016/0009-5907(70)80008-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Ion-exchange resins and inorganic gels have only limited application in the fractionation of DNA. Although DEAE-cellulose has been used extensively, ECTEO-LA-cellulose has promise in the fractionation of DNA. Carboxymethylcellulose or nitrocellulose have limited capabilities as regards DNA fractionation. Gel filtration techniques employing agar gels, DEAE-Sephadex and DEAE-dextran are of more value in the fractionation of s-RNA and polynucleotides than for highly polymerised DNA. Amongst the kieselguhr columns, the methylated serum albumin—kieselguhr column found wide applicability and gives reproducible profiles. An Amberlite IR-120 Al<sup>3+</sup> column also gives interesting results with various potential applications. Alternate methods effecting finer resolution are still a pressing need.</p></div>\",\"PeriodicalId\":78892,\"journal\":{\"name\":\"Chromatographic reviews\",\"volume\":\"12 2\",\"pages\":\"Pages 127-155\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1970-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0009-5907(70)80008-4\",\"citationCount\":\"15\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chromatographic reviews\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0009590770800084\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromatographic reviews","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0009590770800084","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
摘要
离子交换树脂和无机凝胶在DNA分离中的应用有限。虽然deae -纤维素已被广泛使用，但ecteo - la -纤维素在DNA分离方面具有广阔的应用前景。羧甲基纤维素或硝化纤维素在DNA分离方面的能力有限。凝胶过滤技术采用琼脂凝胶，DEAE-Sephadex和DEAE-dextran在s-RNA和多核苷酸的分离中比高度聚合的DNA更有价值。在kieselguhr色谱柱中，甲基化血清白蛋白- kieselguhr色谱柱具有广泛的适用性，并具有可重复性。Amberlite IR-120 Al3+色谱柱也提供了各种潜在应用的有趣结果。实现更精细分辨率的替代方法仍然是迫切需要的。
Present chromatographic methods of fractionating DNA
Ion-exchange resins and inorganic gels have only limited application in the fractionation of DNA. Although DEAE-cellulose has been used extensively, ECTEO-LA-cellulose has promise in the fractionation of DNA. Carboxymethylcellulose or nitrocellulose have limited capabilities as regards DNA fractionation. Gel filtration techniques employing agar gels, DEAE-Sephadex and DEAE-dextran are of more value in the fractionation of s-RNA and polynucleotides than for highly polymerised DNA. Amongst the kieselguhr columns, the methylated serum albumin—kieselguhr column found wide applicability and gives reproducible profiles. An Amberlite IR-120 Al3+ column also gives interesting results with various potential applications. Alternate methods effecting finer resolution are still a pressing need.
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