{"title":"不同年龄大鼠不同器官DNA的体内甲基化。","authors":"D Lutz, M Löwel, H Kröger, D Kubsch, W Uecker","doi":"10.1515/znb-1972-0827","DOIUrl":null,"url":null,"abstract":"We investigated in vivo labelling of DNA in single organs of female Wistar rats after application of 3H-methionine. In 40 g and 120 g rats the tritium incorporation into DNA was 10 times higher in spleen than in liver, kidney and heart. DNA methylation in newborn rats (6 — 8 g) was more pronounced in each of these organs. We demonstrated a varying degree of tritium incorporation into the different bases of DNA depending on the organs. We claim that only the values for 5-methylcytosine should be used as sole criteria of DNA methylation. Our results indi cate that DNA methylation in vivo is enhanced in those organs, where a more intensive DNA synthesis occurs.","PeriodicalId":78857,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie","volume":"27 8","pages":"992-5"},"PeriodicalIF":0.0000,"publicationDate":"1972-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1972-0827","citationCount":"4","resultStr":"{\"title\":\"In vivo methylation of DNA in different organs of rat at various ages.\",\"authors\":\"D Lutz, M Löwel, H Kröger, D Kubsch, W Uecker\",\"doi\":\"10.1515/znb-1972-0827\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We investigated in vivo labelling of DNA in single organs of female Wistar rats after application of 3H-methionine. In 40 g and 120 g rats the tritium incorporation into DNA was 10 times higher in spleen than in liver, kidney and heart. DNA methylation in newborn rats (6 — 8 g) was more pronounced in each of these organs. We demonstrated a varying degree of tritium incorporation into the different bases of DNA depending on the organs. We claim that only the values for 5-methylcytosine should be used as sole criteria of DNA methylation. Our results indi cate that DNA methylation in vivo is enhanced in those organs, where a more intensive DNA synthesis occurs.\",\"PeriodicalId\":78857,\"journal\":{\"name\":\"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie\",\"volume\":\"27 8\",\"pages\":\"992-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1972-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1515/znb-1972-0827\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/znb-1972-0827\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/znb-1972-0827","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In vivo methylation of DNA in different organs of rat at various ages.
We investigated in vivo labelling of DNA in single organs of female Wistar rats after application of 3H-methionine. In 40 g and 120 g rats the tritium incorporation into DNA was 10 times higher in spleen than in liver, kidney and heart. DNA methylation in newborn rats (6 — 8 g) was more pronounced in each of these organs. We demonstrated a varying degree of tritium incorporation into the different bases of DNA depending on the organs. We claim that only the values for 5-methylcytosine should be used as sole criteria of DNA methylation. Our results indi cate that DNA methylation in vivo is enhanced in those organs, where a more intensive DNA synthesis occurs.
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