{"title":"【绿色病变体板层系统发育过程中蛋白质合成的定位】。","authors":"K Lürssen","doi":"10.1515/znb-1971-0718","DOIUrl":null,"url":null,"abstract":"The stromata of etioplasts, greening etioplasts, and chloroplasts contain proteins which crossreact with proteins of the lamellar system of chloroplasts. It is shown that at least one cross-reacting protein is a water-soluble precursor of the lamellar system. The water-insoluble fractions of the plastids were dissolved in buffer containing Na-benzene-dodecylsulfonate and analyzed by gel electrophoresis. The disc electrophoresis patterns of the prolamellar bodies of etioplasts showed 8 protein-containing bands of which 7 were also present in the preparations from greening etioplasts. The latter also yielded 5 new protein-containing bands. The lamellar system of chloroplasts showed only 8 bands which were identical with those of greening etioplasts, but it had only 3 bands in common with the prolamellar bodies of the etioplasts. Two of these proteins may be identical with antigens cross-reacting with stroma proteins.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 7","pages":"725-9"},"PeriodicalIF":0.0000,"publicationDate":"1971-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-0718","citationCount":"5","resultStr":"{\"title\":\"[Localization of protein synthesis during development of the lamellar system of greening etioplasts].\",\"authors\":\"K Lürssen\",\"doi\":\"10.1515/znb-1971-0718\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The stromata of etioplasts, greening etioplasts, and chloroplasts contain proteins which crossreact with proteins of the lamellar system of chloroplasts. It is shown that at least one cross-reacting protein is a water-soluble precursor of the lamellar system. The water-insoluble fractions of the plastids were dissolved in buffer containing Na-benzene-dodecylsulfonate and analyzed by gel electrophoresis. The disc electrophoresis patterns of the prolamellar bodies of etioplasts showed 8 protein-containing bands of which 7 were also present in the preparations from greening etioplasts. The latter also yielded 5 new protein-containing bands. The lamellar system of chloroplasts showed only 8 bands which were identical with those of greening etioplasts, but it had only 3 bands in common with the prolamellar bodies of the etioplasts. Two of these proteins may be identical with antigens cross-reacting with stroma proteins.\",\"PeriodicalId\":23706,\"journal\":{\"name\":\"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete\",\"volume\":\"26 7\",\"pages\":\"725-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1971-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1515/znb-1971-0718\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/znb-1971-0718\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/znb-1971-0718","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Localization of protein synthesis during development of the lamellar system of greening etioplasts].
The stromata of etioplasts, greening etioplasts, and chloroplasts contain proteins which crossreact with proteins of the lamellar system of chloroplasts. It is shown that at least one cross-reacting protein is a water-soluble precursor of the lamellar system. The water-insoluble fractions of the plastids were dissolved in buffer containing Na-benzene-dodecylsulfonate and analyzed by gel electrophoresis. The disc electrophoresis patterns of the prolamellar bodies of etioplasts showed 8 protein-containing bands of which 7 were also present in the preparations from greening etioplasts. The latter also yielded 5 new protein-containing bands. The lamellar system of chloroplasts showed only 8 bands which were identical with those of greening etioplasts, but it had only 3 bands in common with the prolamellar bodies of the etioplasts. Two of these proteins may be identical with antigens cross-reacting with stroma proteins.