大鼠肝脏半胱氨酸磺酸形成酶系统的特点

Lena Ewetz, Bo Sörbo
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引用次数: 68

摘要

1.1. 研究了在羟胺存在下,大鼠肝制剂将半胱氨酸氧化成半胱氨酸磺酸盐。这种化合物被添加到测定系统中是为了抑制酶对反应产物的破坏。报道了该酶体系的最佳测定条件。该反应由可溶性组分中的热不稳定因子催化,并由TPNH、铁离子和线粒体或微粒体刺激。颗粒组分中的刺激活性是热不稳定的。该酶系统似乎对l-半胱氨酸具有特异性,因为当d-半胱氨酸、l-半胱氨酸、谷胱甘肽或半胱氨酸作为底物时,很少检测到亚硫酸盐的形成。在研究的不同大鼠组织中,只有肝脏具有显著活性。该酶体系受重金属试剂(EDTA、氰化物、邻菲罗啉)和巯基试剂对羟基汞苯甲酸酯和碘乙酸酯的抑制,但不受亚砷酸盐的抑制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characteristics of the cysteinesulfinate-forming enzyme system in rat liver

  • 1.

    1. The oxidation of cysteine to cysteinesulfinate by rat-liver preparations in the presence of hydroxylamine has been studied. This compound is added to the assay system in order to inhibit the enzymatic destruction of the reaction product.

  • 2.

    2. The optimum assay conditions for the enzyme system are reported.

  • 3.

    3. The reaction is catalysed by a heat-labile factor found in the soluble fraction and is stimulated by TPNH, ferrous ions and mitochondria or microsomes. The stimulating activity in the particulate fractions is heat-labile.

  • 4.

    4. The enzyme system appears to be specific for l-cysteine, as very little sulfinate formation was detected when d-cysteine, l-cystine, glutathione or cysteamine was used as substrate.

  • 5.

    5. Among different rat tissues studied, only liver contained significant activity.

  • 6.

    6. The enzyme system is inhibited by heavy-metal reagents (EDTA, cyanide, o-phenanthroline) and by the sulfhydryl reagents p-hydroxymercuribenzoate and iodoacetate, but not by arsenite.

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