{"title":"海胆(strongylocentrrotus和centrrostephanus - vi)体腔成分的研究。纤维素-醋酸膜电泳","authors":"Phyllis T Johnson","doi":"10.1016/0010-406X(70)90557-8","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Electrophoretic patterns of proteins and acid mucopolysaccharides found in cell-free coelomic fluid and extracts of ceolomic cells of <em>Strongylocentrotus franciscanus, S. purpuratus</em>, and <em>Centrostephanus coronatus</em> differed according to the urchin species. Most of the proteins were PAS-positive.</p></span></li><li><span>2.</span><span><p>2. In cell-free coelomic fluid, echinochrome A (6-ethyl-2,3,7-trihydroxynaphthazarin) is bound to one or more PAS-positive proteins, according to the urchin species. The same proteins occur in echinochrome-free fluids. In cell extracts echinochrome occurs in PAS-positive proteins that also have an acid-mucopolysaccharide moiety.</p></span></li><li><span>3.</span><span><p>3. Purified echinochrome A conjugated with the proteins ovomucoid and calf-thymus histone, and with macromolecules in fresh cell-free sea-urchin coelomic fluid. It did not conjugate with bovine gamma globulin, or with molecules in concentrated coelomic fluid.</p></span></li></ul></div>","PeriodicalId":78189,"journal":{"name":"Comparative biochemistry and physiology","volume":"37 3","pages":"Pages 289-300"},"PeriodicalIF":0.0000,"publicationDate":"1970-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0010-406X(70)90557-8","citationCount":"12","resultStr":"{\"title\":\"The coelomic elements of sea urchins (Strongylocentrotus and centrostephanus—VI. Cellulose-acetate membrane electrophoresis\",\"authors\":\"Phyllis T Johnson\",\"doi\":\"10.1016/0010-406X(70)90557-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Electrophoretic patterns of proteins and acid mucopolysaccharides found in cell-free coelomic fluid and extracts of ceolomic cells of <em>Strongylocentrotus franciscanus, S. purpuratus</em>, and <em>Centrostephanus coronatus</em> differed according to the urchin species. Most of the proteins were PAS-positive.</p></span></li><li><span>2.</span><span><p>2. In cell-free coelomic fluid, echinochrome A (6-ethyl-2,3,7-trihydroxynaphthazarin) is bound to one or more PAS-positive proteins, according to the urchin species. The same proteins occur in echinochrome-free fluids. In cell extracts echinochrome occurs in PAS-positive proteins that also have an acid-mucopolysaccharide moiety.</p></span></li><li><span>3.</span><span><p>3. Purified echinochrome A conjugated with the proteins ovomucoid and calf-thymus histone, and with macromolecules in fresh cell-free sea-urchin coelomic fluid. It did not conjugate with bovine gamma globulin, or with molecules in concentrated coelomic fluid.</p></span></li></ul></div>\",\"PeriodicalId\":78189,\"journal\":{\"name\":\"Comparative biochemistry and physiology\",\"volume\":\"37 3\",\"pages\":\"Pages 289-300\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1970-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0010-406X(70)90557-8\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative biochemistry and physiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0010406X70905578\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative biochemistry and physiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0010406X70905578","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The coelomic elements of sea urchins (Strongylocentrotus and centrostephanus—VI. Cellulose-acetate membrane electrophoresis
1.
1. Electrophoretic patterns of proteins and acid mucopolysaccharides found in cell-free coelomic fluid and extracts of ceolomic cells of Strongylocentrotus franciscanus, S. purpuratus, and Centrostephanus coronatus differed according to the urchin species. Most of the proteins were PAS-positive.
2.
2. In cell-free coelomic fluid, echinochrome A (6-ethyl-2,3,7-trihydroxynaphthazarin) is bound to one or more PAS-positive proteins, according to the urchin species. The same proteins occur in echinochrome-free fluids. In cell extracts echinochrome occurs in PAS-positive proteins that also have an acid-mucopolysaccharide moiety.
3.
3. Purified echinochrome A conjugated with the proteins ovomucoid and calf-thymus histone, and with macromolecules in fresh cell-free sea-urchin coelomic fluid. It did not conjugate with bovine gamma globulin, or with molecules in concentrated coelomic fluid.
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