{"title":"鸡红细胞AMP脱氨酶与蒽醌类化合物相互作用动力学研究。","authors":"M Yoshino, Y Kawamura","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Alizarine sulfonate, the anthraquinone containing both sulfonate and hydroxyl groups, showed an activating and inhibitory effect on the chicken erythrocyte AMP deaminase (EC 3.5.4.6). The cooperative effect of AMP, analyzed in terms of Hill coefficient, was decreased from 2.4 to 1.1 with the increase in the dye concentration, suggesting the dye as an allosteric activator of the enzyme. However, alizarine sulfonate acted as a mixed type inhibitor in the presence of higher level of AMP. The action of alizarine sulfonate can be accounted for by assuming that the dye binds to the enzyme at the allosteric-activating sites with a broad specificity toward nucleotide binding, and further at the specific inhibitory sites.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":"18 3","pages":"241-4"},"PeriodicalIF":0.0000,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Kinetics of the interactions of the chicken erythrocyte AMP deaminase with anthraquinone compounds.\",\"authors\":\"M Yoshino, Y Kawamura\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Alizarine sulfonate, the anthraquinone containing both sulfonate and hydroxyl groups, showed an activating and inhibitory effect on the chicken erythrocyte AMP deaminase (EC 3.5.4.6). The cooperative effect of AMP, analyzed in terms of Hill coefficient, was decreased from 2.4 to 1.1 with the increase in the dye concentration, suggesting the dye as an allosteric activator of the enzyme. However, alizarine sulfonate acted as a mixed type inhibitor in the presence of higher level of AMP. The action of alizarine sulfonate can be accounted for by assuming that the dye binds to the enzyme at the allosteric-activating sites with a broad specificity toward nucleotide binding, and further at the specific inhibitory sites.</p>\",\"PeriodicalId\":22539,\"journal\":{\"name\":\"The International journal of biochemistry\",\"volume\":\"18 3\",\"pages\":\"241-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1986-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The International journal of biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The International journal of biochemistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Kinetics of the interactions of the chicken erythrocyte AMP deaminase with anthraquinone compounds.
Alizarine sulfonate, the anthraquinone containing both sulfonate and hydroxyl groups, showed an activating and inhibitory effect on the chicken erythrocyte AMP deaminase (EC 3.5.4.6). The cooperative effect of AMP, analyzed in terms of Hill coefficient, was decreased from 2.4 to 1.1 with the increase in the dye concentration, suggesting the dye as an allosteric activator of the enzyme. However, alizarine sulfonate acted as a mixed type inhibitor in the presence of higher level of AMP. The action of alizarine sulfonate can be accounted for by assuming that the dye binds to the enzyme at the allosteric-activating sites with a broad specificity toward nucleotide binding, and further at the specific inhibitory sites.