{"title":"半夏凝集素的放射免疫分析","authors":"Jae-Rok Kim, Kyung-Bae Park, Ok-Doo Awh","doi":"10.1016/0020-708X(85)90252-2","DOIUrl":null,"url":null,"abstract":"<div><p>Radioimmunoassay of <em>Pinellia ternata</em> lectin (PTL) in rabbit serum and in root extract has been tried. The PTL was labelled with <sup>125</sup>I using chloramine-T, and the labelled product was purified by gel chromatography. Standard suppression lines were obtained and the parallelism in serum or buffer was demonstrated. The assay time was 48 h with an intra- and inter-assay coefficient of variation of less than 12%. The PTL content in dried <em>Pinellia ternata</em> roots was about 28±3 mg/g. More than 40% of the i.v. injected PTL to rabbits was retained in serum at 1 h post administration.</p></div>","PeriodicalId":22517,"journal":{"name":"The International journal of applied radiation and isotopes","volume":"36 12","pages":"Pages 943-945"},"PeriodicalIF":0.0000,"publicationDate":"1985-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-708X(85)90252-2","citationCount":"0","resultStr":"{\"title\":\"Radioimmunoassay of Pinellia ternata lectin\",\"authors\":\"Jae-Rok Kim, Kyung-Bae Park, Ok-Doo Awh\",\"doi\":\"10.1016/0020-708X(85)90252-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Radioimmunoassay of <em>Pinellia ternata</em> lectin (PTL) in rabbit serum and in root extract has been tried. The PTL was labelled with <sup>125</sup>I using chloramine-T, and the labelled product was purified by gel chromatography. Standard suppression lines were obtained and the parallelism in serum or buffer was demonstrated. The assay time was 48 h with an intra- and inter-assay coefficient of variation of less than 12%. The PTL content in dried <em>Pinellia ternata</em> roots was about 28±3 mg/g. More than 40% of the i.v. injected PTL to rabbits was retained in serum at 1 h post administration.</p></div>\",\"PeriodicalId\":22517,\"journal\":{\"name\":\"The International journal of applied radiation and isotopes\",\"volume\":\"36 12\",\"pages\":\"Pages 943-945\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1985-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0020-708X(85)90252-2\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The International journal of applied radiation and isotopes\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0020708X85902522\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The International journal of applied radiation and isotopes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0020708X85902522","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Radioimmunoassay of Pinellia ternata lectin (PTL) in rabbit serum and in root extract has been tried. The PTL was labelled with 125I using chloramine-T, and the labelled product was purified by gel chromatography. Standard suppression lines were obtained and the parallelism in serum or buffer was demonstrated. The assay time was 48 h with an intra- and inter-assay coefficient of variation of less than 12%. The PTL content in dried Pinellia ternata roots was about 28±3 mg/g. More than 40% of the i.v. injected PTL to rabbits was retained in serum at 1 h post administration.
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