{"title":"心外膜图像分析使用桌面电脑。心外膜血流分布与nadh荧光模式的比较。","authors":"R Roesen, B Panzner, W Klaus","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>In isolated coronary-ligated rabbit hearts, quantification of epicardial flow distribution pattern and of ischemic area was performed from flow indicator and endogenous NADH-fluorescence pictures, respectively, after UV-flash photography. After being digitized (256 X 256 pixels), the fluorescence photos were analyzed by an Apple desk computer using self-written software (fast machine routines called from BASIC programs). The ischemic area (= sum of pixels with enhanced NADH fluorescence) corresponds to the area with disturbed coronary perfusion (= sum of pixels with diminished indicator fluorescence). Since coronary-active drugs might affect both parameters--local myocardial perfusion and metabolism--differentially, the method presented seems to be a very useful technique for differentiated analysis of the action profile of coronary-active drugs.</p>","PeriodicalId":77831,"journal":{"name":"Advances in myocardiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Epicardial image analysis using a desk top computer. Comparison of epicardial flow distribution and NADH-fluorescence pattern.\",\"authors\":\"R Roesen, B Panzner, W Klaus\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In isolated coronary-ligated rabbit hearts, quantification of epicardial flow distribution pattern and of ischemic area was performed from flow indicator and endogenous NADH-fluorescence pictures, respectively, after UV-flash photography. After being digitized (256 X 256 pixels), the fluorescence photos were analyzed by an Apple desk computer using self-written software (fast machine routines called from BASIC programs). The ischemic area (= sum of pixels with enhanced NADH fluorescence) corresponds to the area with disturbed coronary perfusion (= sum of pixels with diminished indicator fluorescence). Since coronary-active drugs might affect both parameters--local myocardial perfusion and metabolism--differentially, the method presented seems to be a very useful technique for differentiated analysis of the action profile of coronary-active drugs.</p>\",\"PeriodicalId\":77831,\"journal\":{\"name\":\"Advances in myocardiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1985-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in myocardiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in myocardiology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
在离体冠脉结扎兔心脏中,用紫外闪光灯摄影后的血流指示图和内源性nadh荧光图分别定量心外膜血流分布模式和缺血区域。在数字化(256 X 256像素)后,荧光照片由苹果台式电脑使用自己编写的软件(从BASIC程序调用的快速机器例程)进行分析。缺血区域(= NADH荧光增强的像元之和)对应于冠状动脉灌注紊乱区域(=指示荧光减弱的像元之和)。由于冠状动脉活性药物可能对局部心肌灌注和代谢这两个参数产生不同的影响,因此所提出的方法似乎是对冠状动脉活性药物作用谱进行差异化分析的一种非常有用的技术。
Epicardial image analysis using a desk top computer. Comparison of epicardial flow distribution and NADH-fluorescence pattern.
In isolated coronary-ligated rabbit hearts, quantification of epicardial flow distribution pattern and of ischemic area was performed from flow indicator and endogenous NADH-fluorescence pictures, respectively, after UV-flash photography. After being digitized (256 X 256 pixels), the fluorescence photos were analyzed by an Apple desk computer using self-written software (fast machine routines called from BASIC programs). The ischemic area (= sum of pixels with enhanced NADH fluorescence) corresponds to the area with disturbed coronary perfusion (= sum of pixels with diminished indicator fluorescence). Since coronary-active drugs might affect both parameters--local myocardial perfusion and metabolism--differentially, the method presented seems to be a very useful technique for differentiated analysis of the action profile of coronary-active drugs.