磷酸化-去磷酸化对大鼠肝脏胆固醇7 α -羟化酶活性的体外调节的证据:与羟甲基戊二酰辅酶a还原酶的比较。

L Berglund, I Björkhem, B Angelin, K Einarsson
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引用次数: 10

摘要

在有利于磷酸化-去磷酸化的条件下,研究了大鼠肝微粒体中胆固醇7 α -羟化酶的活性。制备过程中,在氟化钠存在或不存在的情况下,酶活性相似。ATP和镁预处理对酶活性没有影响。碱性磷酸酶对胆固醇7 α -羟化酶有抑制作用,但在磷酸酶失活后,这种抑制作用也相似。在类似条件下,大鼠肝脏羟甲基戊二酰辅酶a还原酶活性明显调节,与磷酸化-去磷酸化一致。缺乏对胆固醇7 α -羟化酶的这种调节,反对磷酸化-去磷酸化参与该酶的调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evidence against in vitro modulation of rat liver cholesterol 7 alpha-hydroxylase activity by phosphorylation-dephosphorylation: comparison with hydroxymethylglutaryl CoA reductase.

The activity of cholesterol 7 alpha-hydroxylase in rat liver microsomes was investigated under conditions favourable for phosphorylation-dephosphorylation. The enzyme activity was similar in the presence or absence of sodium fluoride during preparation. Preincubation with ATP and magnesium did not affect the enzyme activity. Cholesterol 7 alpha-hydroxylase was inhibited by alkaline phosphatase, but this inhibition was similar also after inactivation of the phosphatase. Under similar conditions, rat hepatic hydroxymethylglutaryl CoA reductase activity was clearly modulated in agreement with phosphorylation-dephosphorylation. The absence of such a modulation of cholesterol 7 alpha-hydroxylase argues against involvement of phosphorylation-dephosphorylation in the regulation of this enzyme.

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