{"title":"一种利用单克隆抗体定量人血浆中C5a的灵敏ELISA方法。","authors":"M Schulze, O Götze","doi":"10.1159/000467875","DOIUrl":null,"url":null,"abstract":"<p><p>An ELISA for the quantitation of the C5a anaphylatoxin of complement in human plasma is presented which is based on the use of both a monoclonal antibody and polyclonal antibodies to C5a. Its detection limit is 1 ng C5a/ml plasma. It detects no C5a in fresh EDTA-plasma. Complete removal of C5 by an optimized precipitation step prior to the assay procedure is an essential feature of the method.</p>","PeriodicalId":77697,"journal":{"name":"Complement (Basel, Switzerland)","volume":"3 1","pages":"25-39"},"PeriodicalIF":0.0000,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000467875","citationCount":"24","resultStr":"{\"title\":\"A sensitive ELISA for the quantitation of human C5a in blood plasma using a monoclonal antibody.\",\"authors\":\"M Schulze, O Götze\",\"doi\":\"10.1159/000467875\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An ELISA for the quantitation of the C5a anaphylatoxin of complement in human plasma is presented which is based on the use of both a monoclonal antibody and polyclonal antibodies to C5a. Its detection limit is 1 ng C5a/ml plasma. It detects no C5a in fresh EDTA-plasma. Complete removal of C5 by an optimized precipitation step prior to the assay procedure is an essential feature of the method.</p>\",\"PeriodicalId\":77697,\"journal\":{\"name\":\"Complement (Basel, Switzerland)\",\"volume\":\"3 1\",\"pages\":\"25-39\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1986-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1159/000467875\",\"citationCount\":\"24\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Complement (Basel, Switzerland)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1159/000467875\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Complement (Basel, Switzerland)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000467875","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 24
摘要
提出了一种基于C5a单克隆抗体和C5a多克隆抗体的人血浆补体C5a过敏毒素ELISA定量方法。其检出限为1 ng C5a/ml血浆。它在新鲜edta血浆中检测不到C5a。在测定程序之前，通过优化的沉淀步骤完全去除C5是该方法的基本特征。
A sensitive ELISA for the quantitation of human C5a in blood plasma using a monoclonal antibody.
An ELISA for the quantitation of the C5a anaphylatoxin of complement in human plasma is presented which is based on the use of both a monoclonal antibody and polyclonal antibodies to C5a. Its detection limit is 1 ng C5a/ml plasma. It detects no C5a in fresh EDTA-plasma. Complete removal of C5 by an optimized precipitation step prior to the assay procedure is an essential feature of the method.
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