Chih-Wei Liu, Jiahao Feng, Jingya Peng, Haoduo Zhao, Xueying Wang, B. Bhaskar Gollapudi, Abby A. Li, James S. Bus, Christopher Kirman and Kun Lu*,
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Sensitive Quantification of the Ethylene Oxide Hemoglobin Adduct N-(2-Hydroxyethyl)-l-valine from Minimal Blood Volumes
Ethylene oxide (EtO) assessment relies on detecting the hemoglobin adduct N-(2-hydroxyethyl)-l-valine (HE-V). However, current methods require large blood volumes, limiting small-animal exposure studies and reliable detection of endogenous background levels of HE-V. We optimized a highly sensitive analytical workflow including hemoglobin extraction from microliter-scale blood samples, HE-V purification, and targeted LC-MS/MS quantitative analysis. This workflow reliably quantifies endogenous HE-V levels of unexposed mice from only 50 μg of purified hemoglobin (10 μL of blood) and is demonstrated to detect significant HE-V increases at EtO exposures as low as 0.05 ppm. Our platform enables practical EtO biomonitoring in volume-constrained experimental and environmental settings.
期刊介绍：
Chemical Research in Toxicology publishes Articles, Rapid Reports, Chemical Profiles, Reviews, Perspectives, Letters to the Editor, and ToxWatch on a wide range of topics in Toxicology that inform a chemical and molecular understanding and capacity to predict biological outcomes on the basis of structures and processes. The overarching goal of activities reported in the Journal are to provide knowledge and innovative approaches needed to promote intelligent solutions for human safety and ecosystem preservation. The journal emphasizes insight concerning mechanisms of toxicity over phenomenological observations. It upholds rigorous chemical, physical and mathematical standards for characterization and application of modern techniques.
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