通过基因编码的3- cf3 -对苯二胺-赖氨酸的365 nm光触发荧光交联设计，具有红移光除氟作用

Organic chemistry frontiers : an international journal of organic chemistry Pub Date : 2026-06-16 Epub Date: 2026-04-23 DOI:10.1039/d6qo00284f

Fan Zhang , Ziming Xiong , Siyu Kuang , Sitong Li , Xiaohu Zhao , Zhipeng Yu

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引用次数: 0

摘要

我们介绍了3- cf3 -对苯二胺-l -赖氨酸，一种基因可编码的光脱氟-酰基氟交换ncAA。它具有红移活化（311→365 nm），相对于第一代光- dafex ncAA具有更快的光除氟作用，随后Ac-F与R-NH2交联，在可见498nm处产生荧光发射。这有助于捕获和成像在生命系统中的蛋白质-蛋白质相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Design of 365 nm light-triggered fluorogenic crosslinking via a genetically encoded 3-CF3-p-phenylenediamine-lysine featuring red-shifted photo-defluorination

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Design of 365 nm light-triggered fluorogenic crosslinking via a genetically encoded 3-CF3-p-phenylenediamine-lysine featuring red-shifted photo-defluorination

We introduce 3-CF₃-p-phenylenediamine-l-lysine, a genetically encodable photo-defluorination–acyl fluoride exchange ncAA. It features red-shifted activation (311 → 365 nm) for faster photo-defluorination relative to 1^st-generation photo-DAFEx ncAAs, and subsequent Ac-F crosslinking toward R-NH₂ with fluorogenic emission at visible 498 nm. This facilitates capturing and imaging of protein–protein interactions in living systems.

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来源期刊

Organic chemistry frontiers : an international journal of organic chemistry

CiteScore

7.80

自引率

0.00%

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