Sevil Simsek, Cagatay Karaca, Nezihe Koker, Adnan Öztürk, Mustafa Yavuz Koker
{"title":"新生儿和成人的内皮祖细胞数量不同；抗gm- csf α (CD116)抗体作为EPC计数的新标记。","authors":"Sevil Simsek, Cagatay Karaca, Nezihe Koker, Adnan Öztürk, Mustafa Yavuz Koker","doi":"10.1177/17534259261438615","DOIUrl":null,"url":null,"abstract":"<p><p>ObjectiveEndothelial progenitor cells (EPCs) originate from hematopoietic stem cells and can be quantified in peripheral blood using flow cytometry. The anti-GM-CSFα antibody (CD116) may serve as a specific marker for EPC enumeration. This study aimed to quantify peripheral EPCs expressing CD116 and compare the results with other specific antibodies in newborns and adults.Materials and MethodsEPC enumeration was performed by flow cytometric analysis of peripheral blood leukocytes (PBLs) obtained from 50 individuals, including 25 newborns and 25 adults. A CD34-specific antibody was used to identify hematopoietic stem/progenitor cells, while an antibody panel consisting of CD116, CD146, CD31, and CD45 was employed for EPC identification.ResultsEnumeration of CD34<sup>+</sup> hematopoietic progenitor cells (HPCs) demonstrated that the mean CD34<sup>+</sup> HPC count per 10<sup>6</sup> PBLs was 1643 (935-1458) in newborns and 242.7 (163-190) in adults, with a statistically significant difference between the groups (<i>p</i> < 0.001). Using CD146 staining, the mean number of circulating EPCs per 10<sup>6</sup> PBLs was 94.2 (90.5-129.0) in newborns and 9.2 (7.4-12.4) in adults (<i>p</i> < 0.001). Similarly, enumeration based on CD31 staining revealed mean EPC counts of 19.0 (12.5-28.0) in newborns and 6.0 (5.0-7.0) in adults (<i>p</i> < 0.001). Enumeration using CD116 staining showed mean EPC numbers of 29.0 (23.0-34.0) in newborns and 3.0 (2.0-4.0) in adults, also indicating a significant difference between the two groups (<i>p</i> < 0.001).ConclusionEPC numbers are significantly higher in newborns than in adults, suggesting an important developmental role for these cells. The GM-CSFα-specific antibody (CD116) may serve as a novel auxiliary marker for the identification and quantification of circulating EPCsubpopulations. Furthermore, EPC numbers appear to vary across different life stages, with higher numbers in newborns potentially reflecting the presence of a highly regenerative microenvironment.</p>","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"32 ","pages":"17534259261438615"},"PeriodicalIF":2.8000,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13070191/pdf/","citationCount":"0","resultStr":"{\"title\":\"Endothelial progenitor cell numbers vary in newborns and adults; anti-gm-CSFα (CD116) antibody as a novel marker for EPC enumeration.\",\"authors\":\"Sevil Simsek, Cagatay Karaca, Nezihe Koker, Adnan Öztürk, Mustafa Yavuz Koker\",\"doi\":\"10.1177/17534259261438615\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>ObjectiveEndothelial progenitor cells (EPCs) originate from hematopoietic stem cells and can be quantified in peripheral blood using flow cytometry. The anti-GM-CSFα antibody (CD116) may serve as a specific marker for EPC enumeration. This study aimed to quantify peripheral EPCs expressing CD116 and compare the results with other specific antibodies in newborns and adults.Materials and MethodsEPC enumeration was performed by flow cytometric analysis of peripheral blood leukocytes (PBLs) obtained from 50 individuals, including 25 newborns and 25 adults. A CD34-specific antibody was used to identify hematopoietic stem/progenitor cells, while an antibody panel consisting of CD116, CD146, CD31, and CD45 was employed for EPC identification.ResultsEnumeration of CD34<sup>+</sup> hematopoietic progenitor cells (HPCs) demonstrated that the mean CD34<sup>+</sup> HPC count per 10<sup>6</sup> PBLs was 1643 (935-1458) in newborns and 242.7 (163-190) in adults, with a statistically significant difference between the groups (<i>p</i> < 0.001). Using CD146 staining, the mean number of circulating EPCs per 10<sup>6</sup> PBLs was 94.2 (90.5-129.0) in newborns and 9.2 (7.4-12.4) in adults (<i>p</i> < 0.001). Similarly, enumeration based on CD31 staining revealed mean EPC counts of 19.0 (12.5-28.0) in newborns and 6.0 (5.0-7.0) in adults (<i>p</i> < 0.001). Enumeration using CD116 staining showed mean EPC numbers of 29.0 (23.0-34.0) in newborns and 3.0 (2.0-4.0) in adults, also indicating a significant difference between the two groups (<i>p</i> < 0.001).ConclusionEPC numbers are significantly higher in newborns than in adults, suggesting an important developmental role for these cells. The GM-CSFα-specific antibody (CD116) may serve as a novel auxiliary marker for the identification and quantification of circulating EPCsubpopulations. Furthermore, EPC numbers appear to vary across different life stages, with higher numbers in newborns potentially reflecting the presence of a highly regenerative microenvironment.</p>\",\"PeriodicalId\":13676,\"journal\":{\"name\":\"Innate Immunity\",\"volume\":\"32 \",\"pages\":\"17534259261438615\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2026-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13070191/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Innate Immunity\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1177/17534259261438615\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2026/4/10 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Innate Immunity","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1177/17534259261438615","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/4/10 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Endothelial progenitor cell numbers vary in newborns and adults; anti-gm-CSFα (CD116) antibody as a novel marker for EPC enumeration.
ObjectiveEndothelial progenitor cells (EPCs) originate from hematopoietic stem cells and can be quantified in peripheral blood using flow cytometry. The anti-GM-CSFα antibody (CD116) may serve as a specific marker for EPC enumeration. This study aimed to quantify peripheral EPCs expressing CD116 and compare the results with other specific antibodies in newborns and adults.Materials and MethodsEPC enumeration was performed by flow cytometric analysis of peripheral blood leukocytes (PBLs) obtained from 50 individuals, including 25 newborns and 25 adults. A CD34-specific antibody was used to identify hematopoietic stem/progenitor cells, while an antibody panel consisting of CD116, CD146, CD31, and CD45 was employed for EPC identification.ResultsEnumeration of CD34+ hematopoietic progenitor cells (HPCs) demonstrated that the mean CD34+ HPC count per 106 PBLs was 1643 (935-1458) in newborns and 242.7 (163-190) in adults, with a statistically significant difference between the groups (p < 0.001). Using CD146 staining, the mean number of circulating EPCs per 106 PBLs was 94.2 (90.5-129.0) in newborns and 9.2 (7.4-12.4) in adults (p < 0.001). Similarly, enumeration based on CD31 staining revealed mean EPC counts of 19.0 (12.5-28.0) in newborns and 6.0 (5.0-7.0) in adults (p < 0.001). Enumeration using CD116 staining showed mean EPC numbers of 29.0 (23.0-34.0) in newborns and 3.0 (2.0-4.0) in adults, also indicating a significant difference between the two groups (p < 0.001).ConclusionEPC numbers are significantly higher in newborns than in adults, suggesting an important developmental role for these cells. The GM-CSFα-specific antibody (CD116) may serve as a novel auxiliary marker for the identification and quantification of circulating EPCsubpopulations. Furthermore, EPC numbers appear to vary across different life stages, with higher numbers in newborns potentially reflecting the presence of a highly regenerative microenvironment.
期刊介绍：
Innate Immunity is a highly ranked, peer-reviewed scholarly journal and is the official journal of the International Endotoxin & Innate Immunity Society (IEIIS). The journal welcomes manuscripts from researchers actively working on all aspects of innate immunity including biologically active bacterial, viral, fungal, parasitic, and plant components, as well as relevant cells, their receptors, signaling pathways, and induced mediators. The aim of the Journal is to provide a single, interdisciplinary forum for the dissemination of new information on innate immunity in humans, animals, and plants to researchers. The Journal creates a vehicle for the publication of articles encompassing all areas of research, basic, applied, and clinical. The subject areas of interest include, but are not limited to, research in biochemistry, biophysics, cell biology, chemistry, clinical medicine, immunology, infectious disease, microbiology, molecular biology, and pharmacology.
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