{"title":"缺失大肠杆菌6-甲基鸟嘌呤甲基转移酶基因在修复缺陷人细胞中的表达及细胞对烷基化剂抗性的恢复","authors":"Kanji Ishizaki , Tohru Tsujimura , Chikau Fujio , Zhang Yangpei , Hideo Yawata , Yusaku Nakabeppu , Mutsuo Sekiguchi , Mituo Ikenaga","doi":"10.1016/0167-8817(87)90068-X","DOIUrl":null,"url":null,"abstract":"<div><p>We have constructed a truncated <em>E. coli</em><em>O</em><sup>6</sup>-methylguanine methyltransferase (MT) gene (<em>ada</em> gene) to express the MT activity for <em>O</em><sup>6</sup>-methylguanine and <em>O</em><sup>4</sup>-methylthymine but not for methylphosphotriester in human cells and transferred it into Mer<sup>−</sup>HeLle MR cells. The transfectant cells expressed the truncated <em>O</em><sup>6</sup>-<em>E</em>. <em>coli</em> MT were resistant to alkylating agents as same as the transfectant cells with the intact <em>ada</em> gene in cell killing, sister-chromatid exchange induction and host-cell reactivation of adenovirus 5. The results strongly suggest that methylphosphotriester may not contribute to the biological effect of alkylating agents in human cells.</p></div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1987-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(87)90068-X","citationCount":"26","resultStr":"{\"title\":\"Expression of the truncated E. coliO6-methylguanine methyltransferase gene in repair-deficient human cells and restoration of cellular resistance to alkylating agents\",\"authors\":\"Kanji Ishizaki , Tohru Tsujimura , Chikau Fujio , Zhang Yangpei , Hideo Yawata , Yusaku Nakabeppu , Mutsuo Sekiguchi , Mituo Ikenaga\",\"doi\":\"10.1016/0167-8817(87)90068-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We have constructed a truncated <em>E. coli</em><em>O</em><sup>6</sup>-methylguanine methyltransferase (MT) gene (<em>ada</em> gene) to express the MT activity for <em>O</em><sup>6</sup>-methylguanine and <em>O</em><sup>4</sup>-methylthymine but not for methylphosphotriester in human cells and transferred it into Mer<sup>−</sup>HeLle MR cells. The transfectant cells expressed the truncated <em>O</em><sup>6</sup>-<em>E</em>. <em>coli</em> MT were resistant to alkylating agents as same as the transfectant cells with the intact <em>ada</em> gene in cell killing, sister-chromatid exchange induction and host-cell reactivation of adenovirus 5. The results strongly suggest that methylphosphotriester may not contribute to the biological effect of alkylating agents in human cells.</p></div>\",\"PeriodicalId\":100936,\"journal\":{\"name\":\"Mutation Research/DNA Repair Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0167-8817(87)90068-X\",\"citationCount\":\"26\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation Research/DNA Repair Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/016788178790068X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/016788178790068X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Expression of the truncated E. coliO6-methylguanine methyltransferase gene in repair-deficient human cells and restoration of cellular resistance to alkylating agents
We have constructed a truncated E. coliO6-methylguanine methyltransferase (MT) gene (ada gene) to express the MT activity for O6-methylguanine and O4-methylthymine but not for methylphosphotriester in human cells and transferred it into Mer−HeLle MR cells. The transfectant cells expressed the truncated O6-E. coli MT were resistant to alkylating agents as same as the transfectant cells with the intact ada gene in cell killing, sister-chromatid exchange induction and host-cell reactivation of adenovirus 5. The results strongly suggest that methylphosphotriester may not contribute to the biological effect of alkylating agents in human cells.
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