白三烯C4和D4在人尿中的稳定性

G. Beyer, C.O. Meese, U. Klotz
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引用次数: 11

摘要

用实验模型测定了白三烯C4和D4在人尿中的体外稳定性。采用HPLC、液相闪烁计数、RIA、uv -吸光度、GC-MS等方法对各lt进行鉴定和测定。如果新鲜尿液在生理条件下孵育,添加的LTC4分别以时间依赖的方式转化为LTD4 (t12约0.5 h)和LTE4 (t12约6 h)。在22小时的潜伏期结束时,只有不到10%的初始LTC4被检测到。LTB4在这些条件下是稳定的。因此,在实验和临床研究中,在不同条件下收集尿液时,应考虑LTs的尿酶代谢。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Stability of leukotrienes C4 and D4 in human urine

The ex vivo stability of leukotrienes (LT) C4 and D4 in human urine was tested in an experimental model. The various LTs were identified / measured by HPLC, liquid scintillation counting, RIA, UV-absorbance and GC-MS. If fresh urine was incubated under physiological conditions added LTC4 was converted in a time-dependent manner to LTD4 (t12 about 0.5 h) and to LTE4 (t12 about 6 h), respectively. At the end of the 22 h incubation period less than 10 % of the initial LTC4 was detectable. LTB4 was stable under these conditions. Therefore the enzymatic urinary metabolism of LTs should be considered in experimental and clinical studies when urine is collected under different conditions.

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