人胎盘匀浆超滤液中还原剂的抗氧化活性。

N Toh, T Inoue, M Kuraya, H Tanaka, E Kimoto
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引用次数: 0

摘要

通过Sephadex G-25凝胶过滤和DEAE-Sepharose柱层析,从人足月胎盘匀浆超滤液中分离出一种在345 nm处吸收最大而不是在260 nm处吸收最大的还原剂。该物质与烟酰胺核苷酸的还原烟酸部分相似,在氧化作用下,345 nm吸收和457 nm荧光最大值发生衰减,在酸化作用下,吸收最大值从345 nm不可逆地转移到300 nm。与抗坏血酸不同,它的铁细胞色素c还原不依赖于超氧化物。清除芬顿反应产生的羟基自由基。它不促进铁催化的完整和热灭活大鼠肝微粒体脂质过氧化,但抑制NADPH或抗坏血酸介导的微粒体脂质过氧化。在胎盘中,含有高浓度的抗坏血酸和铁离子,345 nm的物质被认为是抗氧化还原剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidative activities of a reductant in the ultrafiltrate of human placental homogenate.

From the ultrafiltrate of human term placental homogenate, a reductant possessing an absorption maximum at 345 nm but not around 260 nm was isolated through the process of Sephadex G-25 gel filtration and DEAE-Sepharose column chromatography. This substance bore resemblance to a reduced nicotinate moiety of nicotinamide nucleotide in regard to a decay in 345 nm absorption and 457 nm fluorescence maximum on oxidation and to an irreversible shift of absorption maximum from 345 nm to 300 nm on acidification. Unlike ascorbate, its ferricytochrome c-reduction was not superoxide-dependent. It scavenged hydroxyl radical produced by Fenton reaction. It did not promote the iron-catalyzed lipid peroxidation of intact and heat-inactivated rat liver microsomes but it inhibited the NADPH or ascorbate-mediated microsomal lipid peroxidation. In the placenta, containing high concentrations of ascorbate and iron ion, 345 nm substance was understood as an antioxidative reductant.

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