丝裂霉素c敏感小鼠淋巴瘤细胞突变体对其他DNA交联剂的生存和致突变反应

Hiroko Hama-Inaba , Koki Sato , Ethel Moustachhi
{"title":"丝裂霉素c敏感小鼠淋巴瘤细胞突变体对其他DNA交联剂的生存和致突变反应","authors":"Hiroko Hama-Inaba ,&nbsp;Koki Sato ,&nbsp;Ethel Moustachhi","doi":"10.1016/0167-8817(88)90014-4","DOIUrl":null,"url":null,"abstract":"<div><p>Mitomycin C-sensitive mutants MCN 151 (complementation group I) and MCE 50 (complementation group II) derived from mouse lymphoma L5178Y cells were found to be also highly sensitive to the lethal effects of other DNA cross-linking agents, such as photoaddition of 8-methoxypsoralen (8-MOP) and <em>cis</em>-diamminedichloroplatinum II (cis-DDP). They were less sensitive to the monofunctional derivative 3-carbethoxypsoralen (3-CPs) and to trans-DDP than their bifunctional counterparts.</p><p>Incorporation levels of labeled 8-MOP or 3-CPs in wild-type cells and 2 mutants were almost the same, indicating that the sensitivity is not caused by differential incorporation of the agents.</p><p>The rates of photoinduced mutations to 6-thioguanine resistance in the mutants, per unit dose of 8-MOP, were about 4 times higher for MNC 151 and 3 times higher for MCE 506 than that in L5178Y cells. However, the rates of induced mutations per viable cells in the mutants were nearly equal to those in wild-type cells.</p><p>Cross-link repair was compared between mutants and wild-type cells by using the alkaline sucrose-gradient gradient sedimentation technique. The results show that normal cells and both mutants are able to incise the cross-linked DNA, which is the fisrst step of cross-link repair.</p></div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(88)90014-4","citationCount":"14","resultStr":"{\"title\":\"Survival and mutagenic responses of mitomycin C-sensitive mouse lymphoma cell mutants to other DNA cross-linking agents\",\"authors\":\"Hiroko Hama-Inaba ,&nbsp;Koki Sato ,&nbsp;Ethel Moustachhi\",\"doi\":\"10.1016/0167-8817(88)90014-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Mitomycin C-sensitive mutants MCN 151 (complementation group I) and MCE 50 (complementation group II) derived from mouse lymphoma L5178Y cells were found to be also highly sensitive to the lethal effects of other DNA cross-linking agents, such as photoaddition of 8-methoxypsoralen (8-MOP) and <em>cis</em>-diamminedichloroplatinum II (cis-DDP). They were less sensitive to the monofunctional derivative 3-carbethoxypsoralen (3-CPs) and to trans-DDP than their bifunctional counterparts.</p><p>Incorporation levels of labeled 8-MOP or 3-CPs in wild-type cells and 2 mutants were almost the same, indicating that the sensitivity is not caused by differential incorporation of the agents.</p><p>The rates of photoinduced mutations to 6-thioguanine resistance in the mutants, per unit dose of 8-MOP, were about 4 times higher for MNC 151 and 3 times higher for MCE 506 than that in L5178Y cells. However, the rates of induced mutations per viable cells in the mutants were nearly equal to those in wild-type cells.</p><p>Cross-link repair was compared between mutants and wild-type cells by using the alkaline sucrose-gradient gradient sedimentation technique. The results show that normal cells and both mutants are able to incise the cross-linked DNA, which is the fisrst step of cross-link repair.</p></div>\",\"PeriodicalId\":100936,\"journal\":{\"name\":\"Mutation Research/DNA Repair Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0167-8817(88)90014-4\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation Research/DNA Repair Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0167881788900144\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0167881788900144","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14

摘要

来自小鼠淋巴瘤L5178Y细胞的丝裂霉素c敏感突变体mcn151(互补组I)和MCE 50(互补组II)也被发现对其他DNA交联剂的致死作用高度敏感,如8-甲氧基补骨脂素(8-MOP)和顺式二胺二氯铂II(顺式ddp)的光作用。它们对单功能衍生物3-碳氧基补骨脂素(3-CPs)和反式ddp的敏感性低于双功能衍生物。标记的8-MOP或3-CPs在野生型细胞和2个突变体中的掺入水平几乎相同,表明敏感性不是由药物掺入差异引起的。单位剂量8-MOP对突变体6-硫鸟嘌呤抗性的光致突变率,MNC 151和MCE 506分别比L5178Y细胞高4倍和3倍。然而,突变体中每个活细胞的诱导突变率几乎与野生型细胞相等。采用碱性蔗糖梯度沉降技术比较了突变体与野生型细胞的交联修复。结果表明,正常细胞和两种突变体都能够切割交联DNA,这是交联修复的第一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Survival and mutagenic responses of mitomycin C-sensitive mouse lymphoma cell mutants to other DNA cross-linking agents

Mitomycin C-sensitive mutants MCN 151 (complementation group I) and MCE 50 (complementation group II) derived from mouse lymphoma L5178Y cells were found to be also highly sensitive to the lethal effects of other DNA cross-linking agents, such as photoaddition of 8-methoxypsoralen (8-MOP) and cis-diamminedichloroplatinum II (cis-DDP). They were less sensitive to the monofunctional derivative 3-carbethoxypsoralen (3-CPs) and to trans-DDP than their bifunctional counterparts.

Incorporation levels of labeled 8-MOP or 3-CPs in wild-type cells and 2 mutants were almost the same, indicating that the sensitivity is not caused by differential incorporation of the agents.

The rates of photoinduced mutations to 6-thioguanine resistance in the mutants, per unit dose of 8-MOP, were about 4 times higher for MNC 151 and 3 times higher for MCE 506 than that in L5178Y cells. However, the rates of induced mutations per viable cells in the mutants were nearly equal to those in wild-type cells.

Cross-link repair was compared between mutants and wild-type cells by using the alkaline sucrose-gradient gradient sedimentation technique. The results show that normal cells and both mutants are able to incise the cross-linked DNA, which is the fisrst step of cross-link repair.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信