{"title":"构建微生物细胞工厂的先进大型DNA操作技术。","authors":"Zubin Pan, Yi Wu","doi":"10.1021/acssynbio.5c00539","DOIUrl":null,"url":null,"abstract":"<p><p>Microbial cell factories play a pivotal role in the field of biomanufacturing. Enhancing their construction efficiency and product yield is essential for advancing synthetic biology and its industrial implementation. In recent years, the rapid development of large DNA manipulation technologies provided powerful support for cloning, assembling, delivering, and rearranging large DNA. This review hence systematically summarizes the core principles and recent advances in four categories of large DNA manipulation techniques. It highlights their key roles in access to complex biosynthetic gene clusters, constructing multigene biosynthetic pathways, introducing complex genetic modules into microbial chassis, structural rewiring, and modular reconstruction for metabolic network optimization. Furthermore, this review explores the emerging trend of large DNA manipulation technologies to advance microbial cell factories. This review is expected to serve as a technical reference for advancing large DNA manipulation technologies and extending their applications toward the construction of high-performance microbial cell factories.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Advanced Large DNA Manipulation Technologies for Constructing Microbial Cell Factories.\",\"authors\":\"Zubin Pan, Yi Wu\",\"doi\":\"10.1021/acssynbio.5c00539\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Microbial cell factories play a pivotal role in the field of biomanufacturing. Enhancing their construction efficiency and product yield is essential for advancing synthetic biology and its industrial implementation. In recent years, the rapid development of large DNA manipulation technologies provided powerful support for cloning, assembling, delivering, and rearranging large DNA. This review hence systematically summarizes the core principles and recent advances in four categories of large DNA manipulation techniques. It highlights their key roles in access to complex biosynthetic gene clusters, constructing multigene biosynthetic pathways, introducing complex genetic modules into microbial chassis, structural rewiring, and modular reconstruction for metabolic network optimization. Furthermore, this review explores the emerging trend of large DNA manipulation technologies to advance microbial cell factories. This review is expected to serve as a technical reference for advancing large DNA manipulation technologies and extending their applications toward the construction of high-performance microbial cell factories.</p>\",\"PeriodicalId\":26,\"journal\":{\"name\":\"ACS Synthetic Biology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-10-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Synthetic Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1021/acssynbio.5c00539\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Synthetic Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1021/acssynbio.5c00539","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Advanced Large DNA Manipulation Technologies for Constructing Microbial Cell Factories.
Microbial cell factories play a pivotal role in the field of biomanufacturing. Enhancing their construction efficiency and product yield is essential for advancing synthetic biology and its industrial implementation. In recent years, the rapid development of large DNA manipulation technologies provided powerful support for cloning, assembling, delivering, and rearranging large DNA. This review hence systematically summarizes the core principles and recent advances in four categories of large DNA manipulation techniques. It highlights their key roles in access to complex biosynthetic gene clusters, constructing multigene biosynthetic pathways, introducing complex genetic modules into microbial chassis, structural rewiring, and modular reconstruction for metabolic network optimization. Furthermore, this review explores the emerging trend of large DNA manipulation technologies to advance microbial cell factories. This review is expected to serve as a technical reference for advancing large DNA manipulation technologies and extending their applications toward the construction of high-performance microbial cell factories.
期刊介绍：
The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism.
Topics may include, but are not limited to:
Design and optimization of genetic systems
Genetic circuit design and their principles for their organization into programs
Computational methods to aid the design of genetic systems
Experimental methods to quantify genetic parts, circuits, and metabolic fluxes
Genetic parts libraries: their creation, analysis, and ontological representation
Protein engineering including computational design
Metabolic engineering and cellular manufacturing, including biomass conversion
Natural product access, engineering, and production
Creative and innovative applications of cellular programming
Medical applications, tissue engineering, and the programming of therapeutic cells
Minimal cell design and construction
Genomics and genome replacement strategies
Viral engineering
Automated and robotic assembly platforms for synthetic biology
DNA synthesis methodologies
Metagenomics and synthetic metagenomic analysis
Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction
Gene optimization
Methods for genome-scale measurements of transcription and metabolomics
Systems biology and methods to integrate multiple data sources
in vitro and cell-free synthetic biology and molecular programming
Nucleic acid engineering.
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