表转录组学分析揭示了补充烟酰胺单核苷酸后人类nad_capped rna的特征。

IF 3.5 3区生物学 Q3 CELL BIOLOGY

Experimental cell research Pub Date : 2025-10-08 DOI:10.1016/j.yexcr.2025.114780

Shuwen Ge , Yandong Liu , Dean Li , Guojing Han , Kongyan Niu , Hujun Ju , Hao Zhou , Rui Liu , Zhengjiang Zhu , Nan Liu , Lefeng Qu

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引用次数: 0

摘要

烟酰胺单核苷酸（NMN）是NAD的前体代谢物，在动物模型中已被证明可以提高细胞NAD水平，并伴有各种与年龄相关的有益作用。nadr -RNA （nadr -RNA）在表转录组水平上代表了一种关键但研究较少的修饰。在这里，我们研究了补充NMN对人外周血单核细胞（pbmc）中nadrna的影响。我们证明，在人类和狗中，NMN的补充增加了NAD的周转率，同时减少了NAD覆盖的rna，揭示了血液来源的NAD- rna是对NMN暴露敏感的潜在生物标志物。

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Epitranscriptomic analysis reveals features of NAD-capped RNAs upon supplementation of nicotinamide mononucleotide in human

Nicotinamide mononucleotide (NMN), a precursory metabolite of NAD, has been demonstrated to boost cellular NAD level that is coupled with various age-related beneficial effects in animal models. NAD-capped RNA (NAD-RNA) represents a critical but poorly studied modification at the epitranscriptomic level. Here we examine the impact of NMN supplementation on NAD-RNA in human peripheral blood mononuclear cells (PBMCs). We demonstrated that NMN supplementation increases NAD turnover coupled with a reduction in NAD-capped RNAs in both human and dog, revealing blood-derived NAD-RNAs as potential biomarkers sensitized to NMN exposure.

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来源期刊

Experimental cell research 医学-细胞生物学

CiteScore

7.20

自引率

0.00%

发文量

295

审稿时长

30 days

期刊介绍： Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.