下丘脑微孔的转录组揭示了北极地鼠冬眠过程中的性别差异。

IF 2.5 4区 生物学 Q3 CELL BIOLOGY
Cole K Deal, M Hoshi Sugiura, Kelly L Drew, Cory T Williams
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引用次数: 0

摘要

季节性的生活史事件,如迁徙、冬眠和繁殖,依赖于协调的生理变化。在脊椎动物中,已知下丘脑中保守的甲状腺激素信号通路触发了许多这些季节性转变。然而,更广泛的过程和调节季节性生理的调节是不明确的。最近对北极地松鼠(AGS, Urocitellus parryii)的研究表明,在冬眠后期,下丘脑甲状腺激素信号被激活,伸长细胞重塑标志物被表达,以期待春季繁殖。我们对冬眠早期和晚期雄性和雌性AGS的下丘脑弓状核、正中隆起、结节部和第三脑室周围的微孔进行了rna测序。我们发现冬眠期间下丘脑转录组存在显著的性别差异。基因表达数据的功能富集分析显示,雌性中与激素转运和神经发生相关的过程和途径上调,而在雄性中则不那么明显。转录因子结合位点分析的差异表达基因鉴定上游调节参与胶质细胞分化,神经元的发育,生存和可塑性。值得注意的是,这些分析中的许多交叉基因都定位于第三脑室底和壁的特化胶质细胞(伸长细胞)。我们的发现支持了一个模型,在这个模型中,基因表达的年度变化依赖于整个冬眠期间伸长细胞的渐进式重塑。这种重塑可能会导致下丘脑神经元可塑性和功能的季节性变化,使大脑在冬眠结束时对生理需求的变化有了预期。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The transcriptomes of hypothalamic micropunches reveal sex differences in regulatory processes across hibernation in the Arctic ground squirrel.

Seasonal life-history events, such as migration, hibernation, and reproduction, depend on coordinated physiological changes. In vertebrates, a conserved thyroid hormone-signaling pathway in the hypothalamus is known to trigger many of these seasonal transitions. However, the broader processes and regulators modulating seasonal physiology are poorly defined. Recent research in Arctic ground squirrels (AGS, Urocitellus parryii) revealed hypothalamic thyroid hormone signaling is activated and markers of tanycytic remodeling are expressed in late hibernation in anticipation of springtime reproduction. We conducted RNA-seq on hypothalamic micropunches encompassing the arcuate nucleus, median eminence, pars tuberalis, and third ventricle in male and female AGS at early and late hibernation. We found substantial sex differences in the hypothalamic transcriptome across hibernation. Functional enrichment analysis of gene expression data revealed an upregulation of processes and pathways related to hormone transport and neurogenesis in females, whereas this was less apparent in males. Transcription factor binding site analysis of differentially expressed genes identified upstream regulators involved in glial cell differentiation, neuronal development, survival, and plasticity. Notably, many of the intersecting genes from these analyses were localized to specialized glial cells (tanycytes) lining the floor and walls of the third ventricle. Our findings support a model in which annual changes in gene expression rely on a progressive remodeling of tanycytes across hibernation. This remodeling may contribute to seasonal changes in neuronal plasticity and function of the hypothalamus, priming the brain in anticipation of shifting physiological demands upon hibernation termination.

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来源期刊
Physiological genomics
Physiological genomics 生物-生理学
CiteScore
6.10
自引率
0.00%
发文量
46
审稿时长
4-8 weeks
期刊介绍: The Physiological Genomics publishes original papers, reviews and rapid reports in a wide area of research focused on uncovering the links between genes and physiology at all levels of biological organization. Articles on topics ranging from single genes to the whole genome and their links to the physiology of humans, any model organism, organ, tissue or cell are welcome. Areas of interest include complex polygenic traits preferably of importance to human health and gene-function relationships of disease processes. Specifically, the Journal has dedicated Sections focused on genome-wide association studies (GWAS) to function, cardiovascular, renal, metabolic and neurological systems, exercise physiology, pharmacogenomics, clinical, translational and genomics for precision medicine, comparative and statistical genomics and databases. For further details on research themes covered within these Sections, please refer to the descriptions given under each Section.
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