哥伦比亚耐药金黄色念珠菌分离株的基因组流行病学研究。

IF 4.5 2区 医学 Q2 MICROBIOLOGY
Maira Lyseth Alvarado Casas, Jeisson Alejandro Triana Díaz, Efrain Montilla-Escudero, Diego Andrés Prada Cardozo, Patricia Escandón Hernández
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引用次数: 0

摘要

自2016年以来,由于其严重的公共卫生影响,哥伦比亚一直对金黄色念珠菌进行强制性报告。在此背景下,通过国家卫生研究所(INS)领导的国家监测,对2018年至2024年间收集的临床auris分离株进行了基因组表征。使用新一代测序(NGS)和生物信息学工具(MycoSNP-nf v1.5、TheiaEuk BLAST、Python script和MUSCLE、snp_phylogeny)对来自14个科室住院患者的27株分离株进行分析,以确定与抗真菌耐药性相关的突变。患者组以男性为主,n = 18(66.7%),老年组(60岁~ 60岁)占48% (n = 13)。抗真菌药敏试验(anidulafungin、氟康唑和两性霉素B)显示5株(18.5%)对一种抗真菌药物耐药,22株(81.5%)对两种抗真菌药物耐药。所有分离株均属于进化支IV(南美洲),遗传差异范围为5 ~ 127个单核苷酸多态性(snp)。在16株分离株中发现了ERG11基因突变,包括Y132F (n = 11)、G459S (n = 1)、K143R (n = 2)和F444L (n = 1),以及一种新的突变G445D (n = 1)。这16株分离株中有4株也携带TAC1B基因突变(P595H, n = 3; S611P, n = 1), 1株分离株携带单一TAC1B突变(S195G)。此外,3株分离株在FKS1基因上出现S639F突变。值得注意的是,7个分离株表现出表型抗性,没有任何已知的抗性突变。这些发现令人担忧,因为所涉及的抗真菌药物对治疗侵袭性念珠菌感染至关重要。该研究强调需要持续的基因组监测来补充表型检测和增强抗真菌耐药性控制策略,最终为治疗决策和药物开发提供信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genomic epidemiology of Colombian resistant Candida auris isolates.

Since 2016, Candida auris has been subject to mandatory reporting in Colombia due to its critical public health implications. In this context, a genomic characterization of clinical C. auris isolates collected between 2018 and 2024 through national surveillance led by the Instituto Nacional de Salud (INS) was conducted. Twenty-seven isolates from hospitalized patients across 14 departments were analyzed using next-generation sequencing (NGS) and bioinformatics tools (MycoSNP-nf v1.5, TheiaEuk BLAST, Python script, and MUSCLE, snp_phylogeny) to identify mutations associated with antifungal resistance. The patient group was predominantly male n = 18 (66.7%), with 48% (n = 13) for the older adult age group (>60 years of age). Antifungal susceptibility testing (anidulafungin, fluconazole, and amphotericin B) revealed that five isolates (18.5%) were resistant to a single antifungal, while 22 (81.5%) were resistant to two. All isolates belonged to clade IV (South America) and exhibited genetic divergence ranging from 5 to 127 single-nucleotide polymorphisms (SNPs). Mutations in the ERG11 gene were identified in 16 isolates, including Y132F (n = 11), G459S (n = 1), K143R (n = 2), and F444L (n = 1), along with a novel mutation, G445D (n = 1). Four of these 16 isolates also harbored mutations in the TAC1B gene (P595H, n = 3; S611P, n = 1), and one isolate carried a single TAC1B mutation (S195G). Additionally, three isolates presented the S639F mutation in the FKS1 gene. Notably, seven isolates showed phenotypic resistance without any known resistance mutations. These findings are concerning, as the antifungals involved are critical for treating invasive Candida infections. The study highlights the need for ongoing genomic surveillance to complement phenotypic testing and enhance strategies for antifungal resistance control, ultimately informing therapeutic decision-making and drug development.

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来源期刊
CiteScore
10.00
自引率
8.20%
发文量
762
审稿时长
3 months
期刊介绍: Antimicrobial Agents and Chemotherapy (AAC) features interdisciplinary studies that build our understanding of the underlying mechanisms and therapeutic applications of antimicrobial and antiparasitic agents and chemotherapy.
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