Sohila G. El-sayed , Hala B. Khalil , Khaled M.A. Ramadan , Khadiga A.A. Abou-Taleb
{"title":"可持续纺织用微生物色素的细胞毒性评价及大鼠皮肤刺激性筛选","authors":"Sohila G. El-sayed , Hala B. Khalil , Khaled M.A. Ramadan , Khadiga A.A. Abou-Taleb","doi":"10.1016/j.aoas.2025.100396","DOIUrl":null,"url":null,"abstract":"<div><div>The global textile industry consumes over a million tons of synthetic dyes annually, posing environmental and health risks due to their persistence and toxicity. Microbial pigments offer a sustainable alternative. This study evaluated the dermal irritation potential of pigments (blue green, brown, red, yellow, rose, and orange) produced by <em>Pseudomonas aeruginosa</em>, <em>Azotobacter chroococcum, Serratia marcescens, Micrococcus luteus, Micrococcus roseus, and Micrococcus kristinae</em> in a rat-based screening model adapted from OECD 404 principles but not fully compliant. No irritation was observed under the tested conditions. Cytotoxicity was assessed using the neutral red uptake assay in WI 38 human lung fibroblasts. The orange pigment was non toxic up to 150 mg/mL, while half-maximal inhibitory concentration (IC₅₀) values for other pigments ranged from 46.7 to 113.5 mg/mL. Dyeing trials on cotton fabric showed the best performance with the red pigment from <em>S. marcescens</em>. This isolate was taxonomically identified by 16S rRNA gene sequencing, which was 99.8 % DNA identical to <em>S. marcescens</em> ATCC13880. The prodigiosin pigment of LRe6 was characterized using Fourier transform infrared spectroscopy. These findings suggest microbial pigments as promising eco-friendly alternatives to synthetic dyes, though further validated testing is required.</div></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"70 2","pages":"Article 100396"},"PeriodicalIF":3.2000,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Assessment of cytotoxicity and rat dermal irritation screening of microbial pigments for application in sustainable textile manufacturing\",\"authors\":\"Sohila G. El-sayed , Hala B. Khalil , Khaled M.A. Ramadan , Khadiga A.A. Abou-Taleb\",\"doi\":\"10.1016/j.aoas.2025.100396\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The global textile industry consumes over a million tons of synthetic dyes annually, posing environmental and health risks due to their persistence and toxicity. Microbial pigments offer a sustainable alternative. This study evaluated the dermal irritation potential of pigments (blue green, brown, red, yellow, rose, and orange) produced by <em>Pseudomonas aeruginosa</em>, <em>Azotobacter chroococcum, Serratia marcescens, Micrococcus luteus, Micrococcus roseus, and Micrococcus kristinae</em> in a rat-based screening model adapted from OECD 404 principles but not fully compliant. No irritation was observed under the tested conditions. Cytotoxicity was assessed using the neutral red uptake assay in WI 38 human lung fibroblasts. The orange pigment was non toxic up to 150 mg/mL, while half-maximal inhibitory concentration (IC₅₀) values for other pigments ranged from 46.7 to 113.5 mg/mL. Dyeing trials on cotton fabric showed the best performance with the red pigment from <em>S. marcescens</em>. This isolate was taxonomically identified by 16S rRNA gene sequencing, which was 99.8 % DNA identical to <em>S. marcescens</em> ATCC13880. The prodigiosin pigment of LRe6 was characterized using Fourier transform infrared spectroscopy. These findings suggest microbial pigments as promising eco-friendly alternatives to synthetic dyes, though further validated testing is required.</div></div>\",\"PeriodicalId\":54198,\"journal\":{\"name\":\"Annals of Agricultural Science\",\"volume\":\"70 2\",\"pages\":\"Article 100396\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-10-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of Agricultural Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0570178325000296\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Agricultural Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0570178325000296","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Assessment of cytotoxicity and rat dermal irritation screening of microbial pigments for application in sustainable textile manufacturing
The global textile industry consumes over a million tons of synthetic dyes annually, posing environmental and health risks due to their persistence and toxicity. Microbial pigments offer a sustainable alternative. This study evaluated the dermal irritation potential of pigments (blue green, brown, red, yellow, rose, and orange) produced by Pseudomonas aeruginosa, Azotobacter chroococcum, Serratia marcescens, Micrococcus luteus, Micrococcus roseus, and Micrococcus kristinae in a rat-based screening model adapted from OECD 404 principles but not fully compliant. No irritation was observed under the tested conditions. Cytotoxicity was assessed using the neutral red uptake assay in WI 38 human lung fibroblasts. The orange pigment was non toxic up to 150 mg/mL, while half-maximal inhibitory concentration (IC₅₀) values for other pigments ranged from 46.7 to 113.5 mg/mL. Dyeing trials on cotton fabric showed the best performance with the red pigment from S. marcescens. This isolate was taxonomically identified by 16S rRNA gene sequencing, which was 99.8 % DNA identical to S. marcescens ATCC13880. The prodigiosin pigment of LRe6 was characterized using Fourier transform infrared spectroscopy. These findings suggest microbial pigments as promising eco-friendly alternatives to synthetic dyes, though further validated testing is required.
期刊介绍:
Annals of Agricultural Sciences (AOAS) is the official journal of Faculty of Agriculture, Ain Shams University. AOAS is an open access peer-reviewed journal publishing original research articles and review articles on experimental and modelling research at laboratory, field, farm, landscape, and industrial levels. AOAS aims to maximize the quality of the agricultural sector across the globe with emphasis on the Arabian countries by focusing on publishing the high-quality applicable researches, in addition to the new methods and frontiers leading to maximizing the quality and quantity of both plant and animal yield and final products.