局部注射PRP对正畸牙齿移动时龈液中RANKL和OPG影响的模型研究

Aida Jafari , Shirin Zahra Farhad , Nasim Esnaashari , Sepehr Kobravi , Hossein Jafari
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引用次数: 0

摘要

目的富血小板血浆(PRP)含有多种生物活性成分,包括生长因子。本研究旨在探讨局部PRP注射对正畸治疗犬龈沟液中骨保护素(OPG)和核因子κ b配体受体激活物(RANKL)介质水平的影响。方法选取6只10 ~ 12月龄成年公犬。每只狗随机分为实验组和对照组,一侧注射凝血酶- cacl2和PRP(实验组),另一侧注射凝血酶- cacl2和安慰剂(对照组)。在实验中,将一侧上颌象限作为实验组,另一侧上颌象限作为对照组。双侧拔除上颌第一前臼齿,然后在第二前臼齿和犬齿之间放置钛镍弹簧(200克)。实验组在第1、21和42天给予PRP和cacl2 -凝血酶的混合物,而对照组则给予cacl2 -凝血酶和安慰剂的混合物。在研究期间(第0、1、2、7、21、42、63天)定期采集龈沟液样本。采用RANKL和OPG酶联免疫吸附法测定培养基的水平。结果实验组的OPG水平持续升高(p < 0.001),表明局部注射PRP对OPG水平及其参与骨重塑的相关调控机制有显著影响。实验组正畸治疗后牙龈液中RANKL介质水平随时间升高,而对照组无明显变化。结论局部注射PRP对OPG和RANKL介质水平有显著影响,重申了其作为优化骨重塑和提高治疗效果的治疗方法的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Locally injected PRP on RANKL and OPG in gingival fluid during orthodontic tooth movement: A Canine Model Study

Aims

Blood platelet-rich plasma (PRP) contains a variety of biologically active components, including growth factors. The objective of this study was to examine the effects of topical PRP injection on osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) mediator levels in the gingival groove fluid of dogs with orthodontic treatment.

Methods

In this study, six adult male dogs, 10–12 months of age, were included. Every dog was randomly assigned to either an experimental or a control group by injecting thrombin-CaCl2 and PRP into one side (experimental group) and thrombin-CaCl2 and a placebo into the opposite side (control group). During the experiment, a maxillary quadrant on one side was assigned as the experimental group and the opposing side as the control group. Maxillary first premolars were bilaterally extracted, followed by the placement of a titanium nickel spring (200 g) between the second premolar and the canine. A mixture of PRP and CaCl2-thrombin was administered to the experimental group on days 1, 21, and 42, whereas the control group received CaCl2-thrombin mixed with a placebo. Samples of gingival groove fluid were collected regularly during the study (on days 0, 1, 2, 7, 21, 42, and 63). A RANKL and OPG enzyme-linked immunosorbent assay was performed to measure the levels of the mediators.

Results

The experimental group exhibited consistently higher OPG levels (p < 0.001), indicating that the topical injection of PRP had a notable effect on OPG levels and the associated regulatory mechanisms involved in bone remodeling. The RANKL mediator levels in the gingival fluid of orthodontic-treated teeth were increased over time in the experimental group, while the control group did not show any significant changes over time.

Conclusion

The topical injection of PRP demonstrated a significant influence on OPG and RANKL mediator levels, reaffirming its potential as a therapeutic approach for optimizing bone remodeling and enhancing treatment outcomes.
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