基于全基因组重测序技术的锦鲤性别决定位点研究

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-09-29 DOI:10.1016/j.cbd.2025.101647

Xu Zhuang , Bin Wen , Zaizhong Chen

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引用次数: 0

摘要

最近通过高通量测序解决了锦鲤（Cyprinus carpio）基因组框架，为性别决定机制提供了新的见解。对锦鲤进行全基因组重测序（WGS），获得1341975个高质量snp。综合分析，包括基因注释，GO功能分类和KEGG途径富集，鉴定了四个核心基因（sox6, wnt7ba， gnrhr4和mrps30）与脊椎动物性别决定途径显著相关。通过PCR-RFLP和Sanger测序验证，gnrhr4外显子区域的性别二态位点SNP3的性别鉴定准确率为100% （n = 20）。本研究不仅为硬骨鱼性别控制育种提供了实用的分子标记，而且对硬骨鱼性别决定的表观遗传调控网络也有了进一步的理论认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A sex-determining loci in koi carp(Cyprinus carpio) based on whole genome resequencing technology

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A sex-determining loci in koi carp(Cyprinus carpio) based on whole genome resequencing technology

The recent resolution of the koi carp (Cyprinus carpio) genome framework through high-throughput sequencing has enabled novel insights into sex determination mechanisms. Whole-genome resequencing (WGS) was performed on koi carp, yielding 1,341,975 high-quality SNPs. Integrated analyses, including gene annotation, GO functional classification, and KEGG pathway enrichment, identified four core genes (sox6, wnt7ba, gnrhr4, and mrps30) significantly associated with vertebrate sex-determination pathways. Validation through PCR-RFLP and Sanger sequencing confirmed that SNP3, a sexually dimorphic locus within the exon region of gnrhr4, demonstrated a sex identification accuracy of 100 % (n = 20).This study not only provides a practical molecular marker for sex-controlled breeding but also advances the theoretical understanding of epigenetic regulatory networks underlying sex determination in teleost fishes.

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.