{"title":"基于苯并喹啉的esipt型荧光探针用于活细胞中次氯酸盐的检测","authors":"Hongwei Zhao, Shijia Guo, Yazhou Jian","doi":"10.1016/j.molliq.2025.128609","DOIUrl":null,"url":null,"abstract":"<div><div>Hypochlorite (ClO<sup>−</sup>), as key reactive oxygen species (ROS), plays significant roles in various pathological and physiological processes. Therefore, we report the design and synthesis of a novel fluorescent probe Bq-CN for the detection of ClO<sup>−</sup>. The probe incorporates 10-hydroxybenzoquinoline as the molecular backbone and diaminomaleonitrile as the recognition group, which reacts with hypochlorite ions to trigger a fluorescence “off-on” response. This probe enables rapid and specific recognition of ClO<sup>−</sup>, and it is not susceptible to interference substances. In addition, it was demonstrated that the probe has low cytotoxicity, can be used for imaging endogenous and exogenous ClO<sup>−</sup> in living cells and zebrafish.</div></div>","PeriodicalId":371,"journal":{"name":"Journal of Molecular Liquids","volume":"437 ","pages":"Article 128609"},"PeriodicalIF":5.2000,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An ESIPT-type fluorescent probe based on benzoquinoline for the detection of hypochlorite in living cells\",\"authors\":\"Hongwei Zhao, Shijia Guo, Yazhou Jian\",\"doi\":\"10.1016/j.molliq.2025.128609\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Hypochlorite (ClO<sup>−</sup>), as key reactive oxygen species (ROS), plays significant roles in various pathological and physiological processes. Therefore, we report the design and synthesis of a novel fluorescent probe Bq-CN for the detection of ClO<sup>−</sup>. The probe incorporates 10-hydroxybenzoquinoline as the molecular backbone and diaminomaleonitrile as the recognition group, which reacts with hypochlorite ions to trigger a fluorescence “off-on” response. This probe enables rapid and specific recognition of ClO<sup>−</sup>, and it is not susceptible to interference substances. In addition, it was demonstrated that the probe has low cytotoxicity, can be used for imaging endogenous and exogenous ClO<sup>−</sup> in living cells and zebrafish.</div></div>\",\"PeriodicalId\":371,\"journal\":{\"name\":\"Journal of Molecular Liquids\",\"volume\":\"437 \",\"pages\":\"Article 128609\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2025-09-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Molecular Liquids\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0167732225017866\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Liquids","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167732225017866","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
An ESIPT-type fluorescent probe based on benzoquinoline for the detection of hypochlorite in living cells
Hypochlorite (ClO−), as key reactive oxygen species (ROS), plays significant roles in various pathological and physiological processes. Therefore, we report the design and synthesis of a novel fluorescent probe Bq-CN for the detection of ClO−. The probe incorporates 10-hydroxybenzoquinoline as the molecular backbone and diaminomaleonitrile as the recognition group, which reacts with hypochlorite ions to trigger a fluorescence “off-on” response. This probe enables rapid and specific recognition of ClO−, and it is not susceptible to interference substances. In addition, it was demonstrated that the probe has low cytotoxicity, can be used for imaging endogenous and exogenous ClO− in living cells and zebrafish.
期刊介绍:
The journal includes papers in the following areas:
– Simple organic liquids and mixtures
– Ionic liquids
– Surfactant solutions (including micelles and vesicles) and liquid interfaces
– Colloidal solutions and nanoparticles
– Thermotropic and lyotropic liquid crystals
– Ferrofluids
– Water, aqueous solutions and other hydrogen-bonded liquids
– Lubricants, polymer solutions and melts
– Molten metals and salts
– Phase transitions and critical phenomena in liquids and confined fluids
– Self assembly in complex liquids.– Biomolecules in solution
The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include:
– Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.)
– Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.)
– Light scattering (Rayleigh, Brillouin, PCS, etc.)
– Dielectric relaxation
– X-ray and neutron scattering and diffraction.
Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.