Maria J.M. Carneiro , Cláudio B.A. Paula , Irisvan S. Ribeiro , Raelle F. Gomes , Jéssica M.T. Souza , José D.B. Marinho Filho , Ana J. Araújo , Rosemayre S. Freire , Jeanlex S. Sousa , R.N. Costa Filho , Judith P.A. Feitosa , Regina C.M. de Paula
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引用次数: 0
摘要
化疗是几种癌症治疗中最广泛使用的方式之一,但单一疗法的有效性通常有限。为了克服这些局限性,研究了纳米载体系统中化疗和光动力治疗(PDT)的结合。在本研究中,制备了与阿霉素(DOX)和姜黄素(CUR)共掺杂的纳米颗粒,用于癌细胞的化学光动力治疗。DOX通过席夫碱形成反应与右旋糖酐-g- pnipam共聚物(CP)结合,而CUR通过非共价相互作用与右旋糖酐-g- pnipam共聚物(CP)结合。CP-DOX和CP-DOX/CUR纳米颗粒具有良好的载药量,且粒径小于90 nm。此外,CP-DOX/CUR纳米颗粒表现出ph依赖的释放曲线,姜黄素化学稳定性测试表明,纳米颗粒可以保护姜黄素免受降解过程的影响。体外细胞毒性实验表明,DOX-和DOX/ cur -负载纳米颗粒可以抑制结肠癌细胞(HCT-116)的增殖,并促进非肿瘤小鼠成纤维细胞的细胞毒性降低(L929)。使用PDT,与负载DOX的纳米颗粒相比,负载DOX/ cur的纳米颗粒具有更大的细胞毒性。因此,负载DOX/ cu的纳米颗粒有望促进药物在癌细胞中的选择性共释放,并在化学光动力治疗中具有潜在的应用前景。
Dextran-based nanoparticles for co-delivery of doxorubicin and curcumin in chemo-photodynamic cancer therapy
Chemotherapy is one of the most widely used modalities in the treatment of several types of cancer, but the effectiveness of monotherapy is generally limited. To overcome these limitations, the combination of chemotherapy and photodynamic therapy (PDT) in nanocarrier systems has been investigated. In this study, nanoparticles co-incorporated with doxorubicin (DOX) and curcumin (CUR) were prepared for chemo-photodynamic therapy in cancer cells. DOX was conjugated to the dextran-g-PNIPAM copolymer (CP) via the Schiff base formation reaction, while CUR was co-incorporated by non-covalent interaction. The CP-DOX and CP-DOX/CUR nanoparticles showed good drug loading and sizes smaller than 90 nm. In addition, the CP-DOX/CUR nanoparticles exhibited pH-dependent release profiles and curcumin chemical stability tests showed that the nanoparticles protected curcumin from degradation processes. In vitro cytotoxicity assays showed that DOX- and DOX/CUR-loaded nanoparticles could inhibit the proliferation of colon cancer cells (HCT-116) and promote reduced cytotoxicity in non-tumor murine fibroblast cells (L929). Using PDT, DOX/CUR-loaded nanoparticles caused greater cytotoxicity compared to DOX-loaded nanoparticles. Therefore, DOX/CUR-loaded nanoparticles are promising to promote selective co-release of drugs in cancer cells and have potential use in chemo-photodynamic therapy.
期刊介绍:
The journal includes papers in the following areas:
– Simple organic liquids and mixtures
– Ionic liquids
– Surfactant solutions (including micelles and vesicles) and liquid interfaces
– Colloidal solutions and nanoparticles
– Thermotropic and lyotropic liquid crystals
– Ferrofluids
– Water, aqueous solutions and other hydrogen-bonded liquids
– Lubricants, polymer solutions and melts
– Molten metals and salts
– Phase transitions and critical phenomena in liquids and confined fluids
– Self assembly in complex liquids.– Biomolecules in solution
The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include:
– Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.)
– Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.)
– Light scattering (Rayleigh, Brillouin, PCS, etc.)
– Dielectric relaxation
– X-ray and neutron scattering and diffraction.
Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.