骨膜蛋白缺失可减少角膜混浊和免疫细胞浸润。

IF 3.6 2区 医学 Q1 PATHOLOGY
Hyemin Seong, Chieun Song, Mingyo Kim, Woong-Sun Yoo, Mee-Young Choi, Réka Dorottya Varga, Yong-Ho Choe, Bina Lee, Seung Pil Yun, Young-Sik Yoo, Youngsub Eom, Choun-Ki Joo, Jinsung Yang, Seong-Jae Kim
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引用次数: 0

摘要

角膜损伤引起的角膜混浊是导致失明的主要原因。细胞外基质(ECM)蛋白、细胞因子和免疫细胞的相互作用诱导角膜损伤后角膜混浊。骨膜蛋白分泌到ECM中,参与伤口愈合并与免疫细胞浸润有关。探讨了骨膜素在角膜创面愈合和角膜混浊形成中的作用。野生型(WT)和Postn敲除(KO)小鼠进行角膜中央切口。WT小鼠切口后骨膜蛋白表达水平显著升高,与纤维连接蛋白、α-SMA等创面愈合标志物水平升高、角膜混浊增加相关。然而,术后KO小鼠显示角膜混浊和免疫细胞浸润减少,尤其是骨髓系细胞。此外,促炎细胞因子(IL-1β、IL-6、C1q)水平在KO后小鼠中没有显著变化。结果表明,骨膜蛋白缺失通过调节细胞因子表达和免疫细胞募集,影响角膜创面愈合,减少角膜不透明。研究结果表明,骨膜蛋白可作为降低角膜混浊的潜在治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Periostin deletion reduces corneal opacity and the infiltration of immune cells.

Corneal opacity resulting from corneal injury is a leading cause of blindness. The interaction of extracellular matrix (ECM) proteins, cytokines and immune cells induces corneal opacity after corneal injury. Periostin, which is secreted into the ECM, is involved in wound healing and is associated with immune cell infiltration. The function of periostin in corneal wound healing and in the development of corneal opacity was investigated. Wild-type (WT) and Postn knockout (KO) mice underwent central corneal incision. Periostin expression level was significantly increased after the incision in WT mice, correlating with higher levels of wound healing markers, such as fibronectin and α-SMA, and increased corneal opacity. However, Postn KO mice showed reduced corneal opacity and immune cell infiltration, particularly from myeloid lineage cells after incision. Additionally, pro-inflammatory cytokine levels (IL-1β, IL-6, C1q) were not significantly changed in Postn KO mice. The results suggest that periostin deletion impairs corneal wound healing and reduces opacity by regulating cytokine expression and immune cell recruitment. The findings indicate that periostin can be a potential therapeutic target for reducing corneal opacity.

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来源期刊
CiteScore
11.40
自引率
0.00%
发文量
178
审稿时长
30 days
期刊介绍: The American Journal of Pathology, official journal of the American Society for Investigative Pathology, published by Elsevier, Inc., seeks high-quality original research reports, reviews, and commentaries related to the molecular and cellular basis of disease. The editors will consider basic, translational, and clinical investigations that directly address mechanisms of pathogenesis or provide a foundation for future mechanistic inquiries. Examples of such foundational investigations include data mining, identification of biomarkers, molecular pathology, and discovery research. Foundational studies that incorporate deep learning and artificial intelligence are also welcome. High priority is given to studies of human disease and relevant experimental models using molecular, cellular, and organismal approaches.
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