丹麦儿童血清神经元特异性烯醇化酶参考间隔及分析前因素的影响。

IF 3.7 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Jacob Rudjord Therkildsen, Cindy Søndersø Knudsen, Tina Parkner
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引用次数: 0

摘要

目的:神经元特异性烯醇化酶(NSE)是一种临床相关的生物标志物,用于评估神经元损伤和某些癌症的诊断和监测。尽管具有重要的诊断意义,但目前缺乏NSE的儿科特异性参考区间(RIs)。本研究旨在建立儿童血清中NSE的RIs,并评估分析前因素对NSE测量的影响。方法:在Cobas平台上使用罗氏Elecsys®NSE检测,对242例丹麦儿童(0.1-17.9岁)常规过敏试验的残留血清样本进行分析。建立了传统的非参数、年龄划分的RIs和通过分位数回归得到的连续RIs。此外,我们评估了分析前变量的影响,包括溶血,并评估了该队列中先前提出的溶血样本校正方法。结果:非参数和连续方法均产生一致的RIs,显示血清NSE浓度随年龄而下降,与性别无关。基于溶血样本,传统的年龄划分RI(第95百分位,单侧)显示0-5岁和6-17岁年龄组血清NSE的上限分别为36.9 和32.0 μg/L。结论:本研究确定了儿童血清NSE的年龄特异性RIs,显示出随年龄增长的生理下降,并强调健康儿童的NSE水平高于成人。此外,在有限的范围内,验证了先前提出的简单线性校正方法,用于使用新建立的RIs调整轻度溶血样品的NSE值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serum neuron-specific enolase - reference interval in Danish children and the impact of preanalytical factors.

Objectives: Neuron-specific enolase (NSE) is a clinically relevant biomarker used in the assessment of neuronal damage and in the diagnosis and monitoring of certain cancers. Despite its diagnostic importance, paediatric-specific reference intervals (RIs) for NSE are currently lacking. This study aimed to establish paediatric RIs for NSE in serum and to evaluate the influence of preanalytical factors on NSE measurements.

Methods: Residual serum samples from routine allergy testing in 242 Danish children (aged 0.1-17.9 years) were analysed using the Roche Elecsys® NSE assay on a Cobas platform. Both traditional non-parametric, age-partitioned RIs and continuous RIs derived via quantile regression were established. In addition, we assessed the impact of preanalytical variables, including haemolysis, and evaluated a previously proposed correction method for haemolysed samples within this cohort.

Results: Both the non-parametric and continuous approaches yielded consistent RIs, showing an age-dependent decline in serum NSE concentrations irrespective of sex. The traditional age-partitioned RI (95th percentile, one-sided) indicated upper limits of 36.9 μg/L and 32.0 μg/L for the age groups 0-5 and 6-17 years of age, based on samples with haemolysis <10 mg/dL haemoglobin.

Conclusions: This study defines age-specific paediatric RIs for serum NSE, demonstrating a physiological decline with age and highlighting higher NSE levels in healthy children compared to adults. Furthermore, within a limited range, a previously proposed simple linear correction method was validated for adjusting NSE values in mildly haemolysed samples using the newly established RIs.

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来源期刊
Clinical chemistry and laboratory medicine
Clinical chemistry and laboratory medicine 医学-医学实验技术
CiteScore
11.30
自引率
16.20%
发文量
306
审稿时长
3 months
期刊介绍: Clinical Chemistry and Laboratory Medicine (CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor over 3. CCLM is issued monthly, and it is published in print and electronically. CCLM is the official journal of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) and publishes regularly EFLM recommendations and news. CCLM is the official journal of the National Societies from Austria (ÖGLMKC); Belgium (RBSLM); Germany (DGKL); Hungary (MLDT); Ireland (ACBI); Italy (SIBioC); Portugal (SPML); and Slovenia (SZKK); and it is affiliated to AACB (Australia) and SFBC (France). Topics: - clinical biochemistry - clinical genomics and molecular biology - clinical haematology and coagulation - clinical immunology and autoimmunity - clinical microbiology - drug monitoring and analysis - evaluation of diagnostic biomarkers - disease-oriented topics (cardiovascular disease, cancer diagnostics, diabetes) - new reagents, instrumentation and technologies - new methodologies - reference materials and methods - reference values and decision limits - quality and safety in laboratory medicine - translational laboratory medicine - clinical metrology Follow @cclm_degruyter on Twitter!
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