来源于嗜盐菌的氧化锌纳米颗粒细胞凋亡介导的抗癌活性研究。

IF 3 4区医学 Q3 CHEMISTRY, MEDICINAL

Anti-cancer agents in medicinal chemistry Pub Date : 2025-09-26 DOI:10.2174/0118715206413187250923055621

Jayasheela Sarilla, Vani Mathakala, Uma Maheswari Devi Palempalli

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引用次数: 0

摘要

简介：纳米医学的最新进展引起了人们对氧化锌纳米颗粒作为细胞凋亡剂治疗三阴性乳腺癌的关注。以二水合乙酸锌为前驱体制备了嗜盐线虫介导的氧化锌纳米颗粒。方法：利用扫描电镜（SEM）和x射线衍射（XRD）对制备的纳米颗粒进行形貌、结构和元素组成表征。以BT-549细胞系为体外模型，采用MTT法和Annexin V-FITC/ pi流式细胞术分析Hb-ZnONPs的抗增殖能力。结果：Hb-ZnONPs在367 nm处表现出特征吸收最大值，粒径为35 nm，稳定性为-44.7 mV。XRD证实了六方纤锌矿结构，元素组成为62.3% Zn和25.79% Oxygen。Hb-ZnONPs对BT-549细胞具有明显的细胞毒性，在5 μg/ml和10 μg/ml浓度下，细胞凋亡率分别为35.26%和38.25%。而凋亡晚期细胞从5 μg/ml浓度下的14.48%增加到10 μg/ml浓度下的28.16%，随着浓度的升高几乎增加了两倍。结论：Hb-ZnONPs可能在乳腺癌化疗中发挥凋亡诱导剂的作用。

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Apoptosis-Mediated Anticancer Activity of Zinc Oxide Nanoparticles Derived and Characterized from Halophila beccarii.

Introduction: Recent advancements in nanomedicine have drawn attention to the use of zinc oxide nanoparticles as apoptotic agents to address triple-negative breast cancer. Halophila beccarii-mediated zinc oxide nanoparticles (Hb-ZnONPs) were fabricated using zinc acetate dihydrate as the precursor.

Methods: The fabricated nanoparticles were characterized based on morphological, structural, and elemental composition using SEM and XRD. The antiproliferative potential of Hb-ZnONPs was studied using the BT-549 cell line as an in vitro model, employing the MTT assay and Annexin V-FITC/PI-based flow cytometry analysis.

Results: The Hb-ZnONPs exhibited characteristic absorption maxima at 367 nm with a particle size of 35 nm and -44.7 mV stability. XRD confirmed the hexagonal wurtzite structure with an elemental composition of 62.3% Zn and 25.79% Oxygen. The Hb-ZnONPs demonstrated significant cytotoxicity against BT-549 cells, with 35.26% apoptosis at 5 μg/ml and 38.25% apoptosis at 10 μg/ml. However, cells in the late apoptosis stage increased from 14.48% at 5 μg/ml to 28.16% at 10 μg/ml, indicating a nearly twofold increase with the higher concentration.

Conclusion: Hb-ZnONPs may act as promising apoptotic inducers in the chemotherapy of breast cancer.

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来源期刊

Anti-cancer agents in medicinal chemistry ONCOLOGY-CHEMISTRY, MEDICINAL

CiteScore

5.10

自引率

3.60%

发文量

323

审稿时长

4-8 weeks

期刊介绍： Formerly: Current Medicinal Chemistry - Anti-Cancer Agents. Anti-Cancer Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of anti-cancer agents. Each issue contains a series of timely in-depth reviews and guest edited issues written by leaders in the field covering a range of current topics in cancer medicinal chemistry. The journal only considers high quality research papers for publication. Anti-Cancer Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in cancer drug discovery.