Huiling Li, Yufei Wei, Zhuoqi Qin, Qiyao Cheng, Yu Hong, Huibin Ru, Tingrui Song, Li Li, Xinzhong Hao, Jianhua Jin, Yan Cheng, Zhifang Wu, Sijin Li
{"title":"18F-FAPI-74正电子发射断层扫描/计算机断层扫描无创监测同种异体心脏移植血管病变纤维化。","authors":"Huiling Li, Yufei Wei, Zhuoqi Qin, Qiyao Cheng, Yu Hong, Huibin Ru, Tingrui Song, Li Li, Xinzhong Hao, Jianhua Jin, Yan Cheng, Zhifang Wu, Sijin Li","doi":"10.1161/JAHA.125.041169","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Cardiac allograft vasculopathy (CAV) is the primary cause of limited long-term survival among heart transplant recipients. This study aimed to evaluate the feasibility of <sup>18</sup>F-FAPI-74 positron emission tomography/computed tomography (PET/CT) imaging for monitoring the fibrosis of CAV.</p><p><strong>Methods: </strong>Rat models of CAV were established and 1 hour dynamic PET/CT imaging was performed in the 15th week postoperation. Serial static <sup>18</sup>F-FAPI-74 PET/CT imaging in the allograft and isograft groups were performed from week 3 to 17. Blocking experiments were performed on allograft rats to confirm the tracer's targeting specificity. In vivo PET/CT imaging findings were further corroborated by ex vivo PET/CT imaging and biodistribution studies. Histological examination was conducted to validate the model's effectiveness, and the correlation between FAP expression levels and imaging results was ascertained.</p><p><strong>Results: </strong>Dynamic PET/CT imaging revealed peak graft heart (Ht) uptake in the allograft group at 60 minutes post <sup>18</sup>F-FAPI-74 injection. PET/CT imaging and biodistribution studies at all time points confirmed significantly higher radioactivity uptake in Ht of the allograft group, as compared with both their native hearts and Ht of the isograft group, and peaking at week 15. There was almost no radioactive accumulation of Ht in the allograft rats blocked by FAPI. Histological analysis confirmed significantly elevated FAP expression in Ht of the allograft group compared with the isograft group, consistent with the severity of CAV.</p><p><strong>Conclusions: </strong>We successfully achieved molecular imaging of activated fibroblasts in CAV rat models, indicating that <sup>18</sup>F-FAPI-74 PET/CT may be a valuable means of evaluating fibrosis progression of CAV.</p>","PeriodicalId":54370,"journal":{"name":"Journal of the American Heart Association","volume":" ","pages":"e041169"},"PeriodicalIF":5.3000,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<sup>18</sup>F-FAPI-74 Positron Emission Tomography/Computed Tomography Noninvasively Monitoring the Fibrosis of Cardiac Allograft Vasculopathy.\",\"authors\":\"Huiling Li, Yufei Wei, Zhuoqi Qin, Qiyao Cheng, Yu Hong, Huibin Ru, Tingrui Song, Li Li, Xinzhong Hao, Jianhua Jin, Yan Cheng, Zhifang Wu, Sijin Li\",\"doi\":\"10.1161/JAHA.125.041169\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Cardiac allograft vasculopathy (CAV) is the primary cause of limited long-term survival among heart transplant recipients. This study aimed to evaluate the feasibility of <sup>18</sup>F-FAPI-74 positron emission tomography/computed tomography (PET/CT) imaging for monitoring the fibrosis of CAV.</p><p><strong>Methods: </strong>Rat models of CAV were established and 1 hour dynamic PET/CT imaging was performed in the 15th week postoperation. Serial static <sup>18</sup>F-FAPI-74 PET/CT imaging in the allograft and isograft groups were performed from week 3 to 17. Blocking experiments were performed on allograft rats to confirm the tracer's targeting specificity. In vivo PET/CT imaging findings were further corroborated by ex vivo PET/CT imaging and biodistribution studies. Histological examination was conducted to validate the model's effectiveness, and the correlation between FAP expression levels and imaging results was ascertained.</p><p><strong>Results: </strong>Dynamic PET/CT imaging revealed peak graft heart (Ht) uptake in the allograft group at 60 minutes post <sup>18</sup>F-FAPI-74 injection. PET/CT imaging and biodistribution studies at all time points confirmed significantly higher radioactivity uptake in Ht of the allograft group, as compared with both their native hearts and Ht of the isograft group, and peaking at week 15. There was almost no radioactive accumulation of Ht in the allograft rats blocked by FAPI. Histological analysis confirmed significantly elevated FAP expression in Ht of the allograft group compared with the isograft group, consistent with the severity of CAV.</p><p><strong>Conclusions: </strong>We successfully achieved molecular imaging of activated fibroblasts in CAV rat models, indicating that <sup>18</sup>F-FAPI-74 PET/CT may be a valuable means of evaluating fibrosis progression of CAV.</p>\",\"PeriodicalId\":54370,\"journal\":{\"name\":\"Journal of the American Heart Association\",\"volume\":\" \",\"pages\":\"e041169\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the American Heart Association\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1161/JAHA.125.041169\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/9/30 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CARDIAC & CARDIOVASCULAR SYSTEMS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Heart Association","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1161/JAHA.125.041169","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/9/30 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CARDIAC & CARDIOVASCULAR SYSTEMS","Score":null,"Total":0}
18F-FAPI-74 Positron Emission Tomography/Computed Tomography Noninvasively Monitoring the Fibrosis of Cardiac Allograft Vasculopathy.
Background: Cardiac allograft vasculopathy (CAV) is the primary cause of limited long-term survival among heart transplant recipients. This study aimed to evaluate the feasibility of 18F-FAPI-74 positron emission tomography/computed tomography (PET/CT) imaging for monitoring the fibrosis of CAV.
Methods: Rat models of CAV were established and 1 hour dynamic PET/CT imaging was performed in the 15th week postoperation. Serial static 18F-FAPI-74 PET/CT imaging in the allograft and isograft groups were performed from week 3 to 17. Blocking experiments were performed on allograft rats to confirm the tracer's targeting specificity. In vivo PET/CT imaging findings were further corroborated by ex vivo PET/CT imaging and biodistribution studies. Histological examination was conducted to validate the model's effectiveness, and the correlation between FAP expression levels and imaging results was ascertained.
Results: Dynamic PET/CT imaging revealed peak graft heart (Ht) uptake in the allograft group at 60 minutes post 18F-FAPI-74 injection. PET/CT imaging and biodistribution studies at all time points confirmed significantly higher radioactivity uptake in Ht of the allograft group, as compared with both their native hearts and Ht of the isograft group, and peaking at week 15. There was almost no radioactive accumulation of Ht in the allograft rats blocked by FAPI. Histological analysis confirmed significantly elevated FAP expression in Ht of the allograft group compared with the isograft group, consistent with the severity of CAV.
Conclusions: We successfully achieved molecular imaging of activated fibroblasts in CAV rat models, indicating that 18F-FAPI-74 PET/CT may be a valuable means of evaluating fibrosis progression of CAV.
期刊介绍:
As an Open Access journal, JAHA - Journal of the American Heart Association is rapidly and freely available, accelerating the translation of strong science into effective practice.
JAHA is an authoritative, peer-reviewed Open Access journal focusing on cardiovascular and cerebrovascular disease. JAHA provides a global forum for basic and clinical research and timely reviews on cardiovascular disease and stroke. As an Open Access journal, its content is free on publication to read, download, and share, accelerating the translation of strong science into effective practice.