Brinley J Klievik, Yan Fu, Aidan D Tyrrell, Chuck T Chen, Adam H Metherel, Richard P Bazinet
{"title":"DHA膳食磷脂载体不会增加脑DHA水平:一项重复研究。","authors":"Brinley J Klievik, Yan Fu, Aidan D Tyrrell, Chuck T Chen, Adam H Metherel, Richard P Bazinet","doi":"10.1016/j.jlr.2025.100913","DOIUrl":null,"url":null,"abstract":"<p><p>DHA is primarily found in fish and seafood as triacylglycerides (TAG) and phospholipids (PL). Oral administration of PL DHA forms, sn-1 lysophosphatidylcholine-DHA (sn-1 LPC-DHA), and di-DHA phosphatidylcholine (di-DHA-PC) have been suggested to increase brain DHA levels by 100% (relative percent) and up to 500% (concentration) compared to controls. In contrast, TAG-DHA and non-esterified (NE)-DHA do not produce increases in brain DHA when provided in the diet. However, a subsequent study using a higher dose of sn-1 LPC-DHA did not confirm these findings and reported no significant increase in brain DHA. To address these inconsistencies, we aimed to replicate previous investigations of PL-DHA forms (LPC and PC) and their impact on brain DHA levels. Mice were randomly divided into one of four groups and received a daily gavage for 30 days of 80 μL of either corn oil alone (control) or corn oil containing 1mg of DHA as NE-DHA, sn-1 LPC-DHA, or di-DHA-PC. DHA relative percent and concentrations were determined in brain regions (cortex, cerebellum, hippocampus, amygdala, striatum, remainder of brain) and plasma using gas chromatography-flame ionization detection. Following treatment, no significant differences in DHA percent or concentration were observed between control and/or treatment groups in any brain region. Relative percent of plasma DHA was significantly elevated in all DHA-treated groups compared to the control group, confirming systemic absorption of the supplemented DHA. Our results demonstrate that dietary DHA provided as sn-1 LPC-DHA or di-DHA-PC does not increase brain DHA levels compared to NE-DHA or the control group, failing to reproduce prior reports.</p>","PeriodicalId":16209,"journal":{"name":"Journal of Lipid Research","volume":" ","pages":"100913"},"PeriodicalIF":4.1000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dietary phospholipid carriers of DHA do not increase brain DHA levels: A replication study.\",\"authors\":\"Brinley J Klievik, Yan Fu, Aidan D Tyrrell, Chuck T Chen, Adam H Metherel, Richard P Bazinet\",\"doi\":\"10.1016/j.jlr.2025.100913\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>DHA is primarily found in fish and seafood as triacylglycerides (TAG) and phospholipids (PL). Oral administration of PL DHA forms, sn-1 lysophosphatidylcholine-DHA (sn-1 LPC-DHA), and di-DHA phosphatidylcholine (di-DHA-PC) have been suggested to increase brain DHA levels by 100% (relative percent) and up to 500% (concentration) compared to controls. In contrast, TAG-DHA and non-esterified (NE)-DHA do not produce increases in brain DHA when provided in the diet. However, a subsequent study using a higher dose of sn-1 LPC-DHA did not confirm these findings and reported no significant increase in brain DHA. To address these inconsistencies, we aimed to replicate previous investigations of PL-DHA forms (LPC and PC) and their impact on brain DHA levels. Mice were randomly divided into one of four groups and received a daily gavage for 30 days of 80 μL of either corn oil alone (control) or corn oil containing 1mg of DHA as NE-DHA, sn-1 LPC-DHA, or di-DHA-PC. DHA relative percent and concentrations were determined in brain regions (cortex, cerebellum, hippocampus, amygdala, striatum, remainder of brain) and plasma using gas chromatography-flame ionization detection. Following treatment, no significant differences in DHA percent or concentration were observed between control and/or treatment groups in any brain region. Relative percent of plasma DHA was significantly elevated in all DHA-treated groups compared to the control group, confirming systemic absorption of the supplemented DHA. Our results demonstrate that dietary DHA provided as sn-1 LPC-DHA or di-DHA-PC does not increase brain DHA levels compared to NE-DHA or the control group, failing to reproduce prior reports.</p>\",\"PeriodicalId\":16209,\"journal\":{\"name\":\"Journal of Lipid Research\",\"volume\":\" \",\"pages\":\"100913\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Lipid Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jlr.2025.100913\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Lipid Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.jlr.2025.100913","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Dietary phospholipid carriers of DHA do not increase brain DHA levels: A replication study.
DHA is primarily found in fish and seafood as triacylglycerides (TAG) and phospholipids (PL). Oral administration of PL DHA forms, sn-1 lysophosphatidylcholine-DHA (sn-1 LPC-DHA), and di-DHA phosphatidylcholine (di-DHA-PC) have been suggested to increase brain DHA levels by 100% (relative percent) and up to 500% (concentration) compared to controls. In contrast, TAG-DHA and non-esterified (NE)-DHA do not produce increases in brain DHA when provided in the diet. However, a subsequent study using a higher dose of sn-1 LPC-DHA did not confirm these findings and reported no significant increase in brain DHA. To address these inconsistencies, we aimed to replicate previous investigations of PL-DHA forms (LPC and PC) and their impact on brain DHA levels. Mice were randomly divided into one of four groups and received a daily gavage for 30 days of 80 μL of either corn oil alone (control) or corn oil containing 1mg of DHA as NE-DHA, sn-1 LPC-DHA, or di-DHA-PC. DHA relative percent and concentrations were determined in brain regions (cortex, cerebellum, hippocampus, amygdala, striatum, remainder of brain) and plasma using gas chromatography-flame ionization detection. Following treatment, no significant differences in DHA percent or concentration were observed between control and/or treatment groups in any brain region. Relative percent of plasma DHA was significantly elevated in all DHA-treated groups compared to the control group, confirming systemic absorption of the supplemented DHA. Our results demonstrate that dietary DHA provided as sn-1 LPC-DHA or di-DHA-PC does not increase brain DHA levels compared to NE-DHA or the control group, failing to reproduce prior reports.
期刊介绍:
The Journal of Lipid Research (JLR) publishes original articles and reviews in the broadly defined area of biological lipids. We encourage the submission of manuscripts relating to lipids, including those addressing problems in biochemistry, molecular biology, structural biology, cell biology, genetics, molecular medicine, clinical medicine and metabolism. Major criteria for acceptance of articles are new insights into mechanisms of lipid function and metabolism and/or genes regulating lipid metabolism along with sound primary experimental data. Interpretation of the data is the authors’ responsibility, and speculation should be labeled as such. Manuscripts that provide new ways of purifying, identifying and quantifying lipids are invited for the Methods section of the Journal. JLR encourages contributions from investigators in all countries, but articles must be submitted in clear and concise English.