DHA膳食磷脂载体不会增加脑DHA水平:一项重复研究。

IF 4.1 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Brinley J Klievik, Yan Fu, Aidan D Tyrrell, Chuck T Chen, Adam H Metherel, Richard P Bazinet
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引用次数: 0

摘要

DHA主要以甘油三酯(TAG)和磷脂(PL)的形式存在于鱼类和海鲜中。与对照组相比,口服PL DHA形式,sn-1溶血磷脂酰胆碱-DHA (sn-1 LPC-DHA)和双DHA磷脂酰胆碱(di-DHA- pc)被认为可以使脑DHA水平增加100%(相对百分比)和高达500%(浓度)。相反,当饮食中提供TAG-DHA和非酯化(NE)-DHA时,不会产生脑DHA的增加。然而,随后一项使用更高剂量sn-1 LPC-DHA的研究并没有证实这些发现,也没有报告大脑DHA的显著增加。为了解决这些不一致,我们的目标是复制先前对PL-DHA形式(LPC和PC)及其对大脑DHA水平影响的研究。将小鼠随机分为4组,每天分别灌胃80 μL玉米油(对照组)或含有1mg DHA的玉米油(NE-DHA、sn-1 LPC-DHA或di-DHA-PC),连续30 d。采用气相色谱-火焰电离检测法测定脑区(皮层、小脑、海马、杏仁核、纹状体、其余脑区)和血浆中DHA的相对百分比和浓度。治疗后,在对照组和/或治疗组之间的任何脑区DHA百分比或浓度均无显著差异。与对照组相比,所有DHA处理组血浆DHA的相对百分比显著升高,证实了补充DHA的全身吸收。我们的研究结果表明,与NE-DHA或对照组相比,以sn-1 LPC-DHA或di-DHA-PC形式提供的膳食DHA不会增加大脑DHA水平,这未能重现先前的报道。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dietary phospholipid carriers of DHA do not increase brain DHA levels: A replication study.

DHA is primarily found in fish and seafood as triacylglycerides (TAG) and phospholipids (PL). Oral administration of PL DHA forms, sn-1 lysophosphatidylcholine-DHA (sn-1 LPC-DHA), and di-DHA phosphatidylcholine (di-DHA-PC) have been suggested to increase brain DHA levels by 100% (relative percent) and up to 500% (concentration) compared to controls. In contrast, TAG-DHA and non-esterified (NE)-DHA do not produce increases in brain DHA when provided in the diet. However, a subsequent study using a higher dose of sn-1 LPC-DHA did not confirm these findings and reported no significant increase in brain DHA. To address these inconsistencies, we aimed to replicate previous investigations of PL-DHA forms (LPC and PC) and their impact on brain DHA levels. Mice were randomly divided into one of four groups and received a daily gavage for 30 days of 80 μL of either corn oil alone (control) or corn oil containing 1mg of DHA as NE-DHA, sn-1 LPC-DHA, or di-DHA-PC. DHA relative percent and concentrations were determined in brain regions (cortex, cerebellum, hippocampus, amygdala, striatum, remainder of brain) and plasma using gas chromatography-flame ionization detection. Following treatment, no significant differences in DHA percent or concentration were observed between control and/or treatment groups in any brain region. Relative percent of plasma DHA was significantly elevated in all DHA-treated groups compared to the control group, confirming systemic absorption of the supplemented DHA. Our results demonstrate that dietary DHA provided as sn-1 LPC-DHA or di-DHA-PC does not increase brain DHA levels compared to NE-DHA or the control group, failing to reproduce prior reports.

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来源期刊
Journal of Lipid Research
Journal of Lipid Research 生物-生化与分子生物学
CiteScore
11.10
自引率
4.60%
发文量
146
审稿时长
41 days
期刊介绍: The Journal of Lipid Research (JLR) publishes original articles and reviews in the broadly defined area of biological lipids. We encourage the submission of manuscripts relating to lipids, including those addressing problems in biochemistry, molecular biology, structural biology, cell biology, genetics, molecular medicine, clinical medicine and metabolism. Major criteria for acceptance of articles are new insights into mechanisms of lipid function and metabolism and/or genes regulating lipid metabolism along with sound primary experimental data. Interpretation of the data is the authors’ responsibility, and speculation should be labeled as such. Manuscripts that provide new ways of purifying, identifying and quantifying lipids are invited for the Methods section of the Journal. JLR encourages contributions from investigators in all countries, but articles must be submitted in clear and concise English.
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