不同量产技术对提高印度喜马拉雅地区原生昆虫病原线虫分离株后代的评价

IF 1.3 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Simranjeet Kaur, Neelam Thakur, Ajar Nath Yadav
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引用次数: 0

摘要

利用本地昆虫病原线虫(EPNs)作为生物防治剂,可以代替环境稳定性,提供全面虫害管理的最佳途径,其益处广泛且对其他生物的副作用小。这些有益线虫的鉴定和大规模培养是田间应用的关键。EPNs种群可作为生物农药配方和田间喷雾应用的限制因素。本研究旨在利用体内和体外批量生产技术,克服土壤中昆虫病原线虫数量少的局限性。从喜马偕尔邦索兰地区分离到一株异habditis,并在实验室进行了大规模培养。在体内技术中,采用改良白诱法,以大蜡蛾(Galleria mellonella Linnaeus)和米蛾(Corcyra cephalonica Stainton)幼虫为诱饵昆虫,改良WM(1 ~ 200万种群范围)3 d内可回收较多的感染幼虫(IJs), DBM的最大子代产量在2 ~ 300万种群范围内,出蛹时间为7 d。本研究表明,利用不同的批量生产技术可以有效地抑制mellonella Galleria (23100 ij) epn数量低于cephalonica Corcyra (12840 ij) epn数量的限制。采用改良犬饼干培养基(DBM)、改良kabuli克培养基(KGM)、黑克培养基(BGM)和改良wout培养基(WM)进行线虫离体批量生产。改良WM(1 ~ 200万种群)最小羽化时间为3 d, DBM羽化时间为7 d,最大羽化时间为2 ~ 300万种群。初步分离和小批量生产采用体内培养的方法效果最好,蜡蛾是较好的饵料昆虫。体外技术的应用有利于分离物的大量增殖,便于现场应用。本研究为推广昆虫病原线虫作为生物防治剂和化学农药替代品提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Different Mass Production Techniques to Enhance the Progeny of Isolated Indigenous Entomopathogenic Nematodes from Indian Himalayan Region

The use of indigenous entomopathogenic nematodes (EPNs) as biological control agent can provide the best way for overall insect pest management in lieu of environmental stability with their wide range of benefits and less side-effect on other organisms. The identification and mass culturing are pivotal for field applications of these beneficial nematodes. The EPNs population can serve as a limiting factor in the biopesticides formulation and field spray application. This study was formulated to curb out the limitation of low population of entomopathogenic nematodes in the soil using the in vivo and in vitro mass production techniques. An indigenous strain of genus Heterorhabditis was isolated from Solan district of Himachal Pradesh and then mass cultured in the laboratory. In in vivo technique, larvae of Galleria mellonella Linnaeus (greater wax moth) and Corcyra cephalonica Stainton (rice moth) were used as bait insects in modified method of white trap and as a result, a greater number of infective juvenile (IJs) were recovered of IJs were 3 days in modified WM (1–2 million population ranges) and the maximum progeny production within the range of 2–3 million was observed on DBM with emergence time of 7 days. The present investigation suggests that the utilization of different mass production techniques can curb the constraint of low population of EPNs from, Galleria mellonella (23100 IJs) as compared to Corcyra cephalonica (12840 IJs). The culture media, including modified dog biscuit medium (DBM), modified kabuli gram medium (KGM), black gram medium (BGM) and modified wout medium (WM) were used for the in vitro mass production of isolated nematodes. The minimum time taken for emergence of IJs were 3 days in modified WM (1–2 million population ranges) and the maximum progeny production within the range of 2–3 million was observed on DBM with emergence time of 7 days. The use of in vivo culturing was found to be best for initial isolation and small-scale mass production and greater wax moth is better bait insect in comparison to rice moth. The use of in vitro technique was helpful in mass multiplication in large number of the isolate, therefore making it convenient for field application. The present study could help in popularizing the use of entomopathogenic nematodes as biological control agents and alternative to chemical pesticides.

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来源期刊
National Academy Science Letters
National Academy Science Letters 综合性期刊-综合性期刊
CiteScore
2.20
自引率
0.00%
发文量
86
审稿时长
12 months
期刊介绍: The National Academy Science Letters is published by the National Academy of Sciences, India, since 1978. The publication of this unique journal was started with a view to give quick and wide publicity to the innovations in all fields of science
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