E. A. Molkova, T. A. Matveeva, I. V. Baimler, R. M. Sarimov, S. V. Gudkov, A. S. Dorokhov, A. Yu. Izmailov
{"title":"光学方法研究水溶液pH和温度对金纳米颗粒-溶菌酶蛋白体系稳定性的影响","authors":"E. A. Molkova, T. A. Matveeva, I. V. Baimler, R. M. Sarimov, S. V. Gudkov, A. S. Dorokhov, A. Yu. Izmailov","doi":"10.3103/S1541308X25700141","DOIUrl":null,"url":null,"abstract":"<p>Gold nanoparticles are widely used in spectroscopy for detecting/studying substances in biological media. Such particles in biological objects inevitably interact with proteins, which obviously affects the functions and properties of the nanomaterial. The influence of external conditions on the interaction of nanoparticles with proteins in the aqueous phase has still remained little studied. In this paper we investigate the influence of temperature and pH of the medium on the interaction of lysozyme protein with gold nanoparticles in aqueous solutions. It is found that the temperature of the medium affects the size of aggregates of gold nanoparticles with protein at pH 7.5 of the aqueous solutions. It is found that lysozyme prevents protein aggregation at acidic pH values; with an increase in temperature, gold particles with a lysozyme protein crown retain their size. The results of this study gain a deeper insight into the processes of interaction between enzymes and gold nanoparticles with a change in the solvent properties.</p>","PeriodicalId":732,"journal":{"name":"Physics of Wave Phenomena","volume":"33 3","pages":"192 - 206"},"PeriodicalIF":1.1000,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of the Influence of pH and Temperature of an Aqueous Solution on the Stability of the Gold Nanoparticles–Lysozyme Protein System by Optical Methods\",\"authors\":\"E. A. Molkova, T. A. Matveeva, I. V. Baimler, R. M. Sarimov, S. V. Gudkov, A. S. Dorokhov, A. Yu. Izmailov\",\"doi\":\"10.3103/S1541308X25700141\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Gold nanoparticles are widely used in spectroscopy for detecting/studying substances in biological media. Such particles in biological objects inevitably interact with proteins, which obviously affects the functions and properties of the nanomaterial. The influence of external conditions on the interaction of nanoparticles with proteins in the aqueous phase has still remained little studied. In this paper we investigate the influence of temperature and pH of the medium on the interaction of lysozyme protein with gold nanoparticles in aqueous solutions. It is found that the temperature of the medium affects the size of aggregates of gold nanoparticles with protein at pH 7.5 of the aqueous solutions. It is found that lysozyme prevents protein aggregation at acidic pH values; with an increase in temperature, gold particles with a lysozyme protein crown retain their size. The results of this study gain a deeper insight into the processes of interaction between enzymes and gold nanoparticles with a change in the solvent properties.</p>\",\"PeriodicalId\":732,\"journal\":{\"name\":\"Physics of Wave Phenomena\",\"volume\":\"33 3\",\"pages\":\"192 - 206\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-07-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physics of Wave Phenomena\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://link.springer.com/article/10.3103/S1541308X25700141\",\"RegionNum\":4,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHYSICS, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physics of Wave Phenomena","FirstCategoryId":"101","ListUrlMain":"https://link.springer.com/article/10.3103/S1541308X25700141","RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHYSICS, MULTIDISCIPLINARY","Score":null,"Total":0}
Investigation of the Influence of pH and Temperature of an Aqueous Solution on the Stability of the Gold Nanoparticles–Lysozyme Protein System by Optical Methods
Gold nanoparticles are widely used in spectroscopy for detecting/studying substances in biological media. Such particles in biological objects inevitably interact with proteins, which obviously affects the functions and properties of the nanomaterial. The influence of external conditions on the interaction of nanoparticles with proteins in the aqueous phase has still remained little studied. In this paper we investigate the influence of temperature and pH of the medium on the interaction of lysozyme protein with gold nanoparticles in aqueous solutions. It is found that the temperature of the medium affects the size of aggregates of gold nanoparticles with protein at pH 7.5 of the aqueous solutions. It is found that lysozyme prevents protein aggregation at acidic pH values; with an increase in temperature, gold particles with a lysozyme protein crown retain their size. The results of this study gain a deeper insight into the processes of interaction between enzymes and gold nanoparticles with a change in the solvent properties.
期刊介绍：
Physics of Wave Phenomena publishes original contributions in general and nonlinear wave theory, original experimental results in optics, acoustics and radiophysics. The fields of physics represented in this journal include nonlinear optics, acoustics, and radiophysics; nonlinear effects of any nature including nonlinear dynamics and chaos; phase transitions including light- and sound-induced; laser physics; optical and other spectroscopies; new instruments, methods, and measurements of wave and oscillatory processes; remote sensing of waves in natural media; wave interactions in biophysics, econophysics and other cross-disciplinary areas.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
