Xianhong Zhu, Min Zhang, Kunming Qin, Xueying Qin, Miaomiao Chi, Yan Zhang, Hongsen Wang, Xichen Song, Xun Gao
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引用次数: 0
摘要
腋窝脊柱炎（cherospondias axillaris， C. axillaris）是公认的治疗冠心病（CHD）的有效草药，已被临床应用。尽管血管紧张素转换酶（ACE）作为冠心病治疗的相关靶点已被广泛研究，但目前关于从腋窝草中筛选潜在ACE抑制剂的研究有限。为了促进腋毛在冠心病治疗中的更广泛应用，本研究将磁靶向捕捞技术与功能化磁纳米球相结合，对腋毛中ACE的靶活性成分进行了分析。利用振动样品磁强计（VSM）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）对GO@Fe3O4@SiO2-ACE （SMGO-ACE）功能化的磁性纳米球进行了表征。利用赖诺普利与血管紧张素转换酶的特异性结合，优化了合成材料的性能和靶向捕捞的相关条件。随后，鉴定出5种有效成分为(2R,3S)-二氢脱氢松叶醇、异香兰素、奎宁酸、菊花素和异鼠李素。本研究为复杂混合物中有效成分的定向提取分离提供了先例。
Affinity Fishing of ACE Ligands from C. axillaris using Surface Functionalized Magnetic Nanoparticles
Choerospondias axillaris (C. axillaris), recognized as an effective herbal remedy for coronary heart disease (CHD), has been clinically utilized. Although the angiotensin-converting enzyme (ACE) has been extensively investigated as a pertinent target for CHD treatment, there is currently limited research on screening potential ACE inhibitors from C. axillaris. To advance the broader utilization of C. axillaris in coronary heart disease treatment, the target active components of ACE in C. axillaris were analyzed through the integration of magnetic-targeted fishing technology with functionalized magnetic nanospheres. Magnetic nanospheres functionalized with GO@Fe3O4@SiO2-ACE (SMGO-ACE) were characterized using a vibrating sample magnetometer (VSM), scanning electron microscopy (SEM), and transmission electron microscope (TEM). The specific binding of lisinopril with the angiotensin-converting enzyme was employed to optimize the performance of the synthesized material and the relevant conditions during targeted fishing. Subsequently, five active ingredients were identified as (2R,3S)-Dihydrodehydroconiferyl alcohol, isovanillin, quinic acid, chrysin, and isorhamnetin. This study provides a precedent for the targeted extraction and separation of active ingredients in complex mixtures.
期刊介绍：
Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.
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