Ebtesam W. Elsayed, Maha F. Emam, Ahmed A. El-Ashmawy, Gehan T. El-Bassyouni, Sahar M. Mousa, Ahmed A. F. Soliman, M. El-Manawaty, Laila H. Emara
{"title":"含有5-氟尿嘧啶和锌修饰羟基磷灰石增强的有前途的海藻酸盐-明胶支架：细胞附着和一年的稳定性研究","authors":"Ebtesam W. Elsayed, Maha F. Emam, Ahmed A. El-Ashmawy, Gehan T. El-Bassyouni, Sahar M. Mousa, Ahmed A. F. Soliman, M. El-Manawaty, Laila H. Emara","doi":"10.1007/s12247-025-10032-5","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>The current study evaluated the cell attachment capability and long-term stability of 5<b>-</b>fluorouracil (5-FU) loaded alginate-gelatin scaffolds reinforced with zinc-doped hydroxyapatite (Zn-HA).</p><h3>Methods</h3><p>Zn-HA (2% and 6% Zn) was prepared and evaluated for the crystalline nature, chemical structure, and microstructure of hydroxyapatite (HA). Blank and 5-FU loaded alginate-gelatin-Zn-HA scaffolds were prepared and evaluated in terms of surface area, pore size, pore volume, and morphology using the gas adsorption technique and scanning electron microscopy (SEM). Cell attachment studies were also carried out on normal human cell line. The scaffolds were stored for one year at room temperature and evaluated for any changes in their physico-chemical properties as well as 5-FU release profile.</p><h3>Results</h3><p>The results showed that increasing zinc content decreased HA particle size. The blank scaffolds showed higher surface area, pore volume values, and a less porous structure compared to the drug-loaded ones. The pore size increased after scaffolds soaking in cell-line medium due to the hydrogel-forming materials' biodegradability and drug release. Cell growth on the presoaked drug-loaded scaffolds was improved compared to blank scaffold. XRD, FTIR and 5-FU release profile indicated that the stored scaffolds did not show any physical or chemical changes.</p><h3>Conclusion</h3><p>The prepared scaffolds showed promising results regarding cell attachment capability and long-term stability.</p></div>","PeriodicalId":656,"journal":{"name":"Journal of Pharmaceutical Innovation","volume":"20 4","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12247-025-10032-5.pdf","citationCount":"0","resultStr":"{\"title\":\"Promising Alginate-Gelatin Scaffolds Containing 5-Fluorouracil and Reinforced with Zinc-Modified Hydroxyapatite: Cell Attachment and One-Year Stability Study\",\"authors\":\"Ebtesam W. Elsayed, Maha F. Emam, Ahmed A. El-Ashmawy, Gehan T. El-Bassyouni, Sahar M. Mousa, Ahmed A. F. Soliman, M. El-Manawaty, Laila H. Emara\",\"doi\":\"10.1007/s12247-025-10032-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>The current study evaluated the cell attachment capability and long-term stability of 5<b>-</b>fluorouracil (5-FU) loaded alginate-gelatin scaffolds reinforced with zinc-doped hydroxyapatite (Zn-HA).</p><h3>Methods</h3><p>Zn-HA (2% and 6% Zn) was prepared and evaluated for the crystalline nature, chemical structure, and microstructure of hydroxyapatite (HA). Blank and 5-FU loaded alginate-gelatin-Zn-HA scaffolds were prepared and evaluated in terms of surface area, pore size, pore volume, and morphology using the gas adsorption technique and scanning electron microscopy (SEM). Cell attachment studies were also carried out on normal human cell line. The scaffolds were stored for one year at room temperature and evaluated for any changes in their physico-chemical properties as well as 5-FU release profile.</p><h3>Results</h3><p>The results showed that increasing zinc content decreased HA particle size. The blank scaffolds showed higher surface area, pore volume values, and a less porous structure compared to the drug-loaded ones. The pore size increased after scaffolds soaking in cell-line medium due to the hydrogel-forming materials' biodegradability and drug release. Cell growth on the presoaked drug-loaded scaffolds was improved compared to blank scaffold. XRD, FTIR and 5-FU release profile indicated that the stored scaffolds did not show any physical or chemical changes.</p><h3>Conclusion</h3><p>The prepared scaffolds showed promising results regarding cell attachment capability and long-term stability.</p></div>\",\"PeriodicalId\":656,\"journal\":{\"name\":\"Journal of Pharmaceutical Innovation\",\"volume\":\"20 4\",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s12247-025-10032-5.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pharmaceutical Innovation\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s12247-025-10032-5\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pharmaceutical Innovation","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s12247-025-10032-5","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Promising Alginate-Gelatin Scaffolds Containing 5-Fluorouracil and Reinforced with Zinc-Modified Hydroxyapatite: Cell Attachment and One-Year Stability Study
Purpose
The current study evaluated the cell attachment capability and long-term stability of 5-fluorouracil (5-FU) loaded alginate-gelatin scaffolds reinforced with zinc-doped hydroxyapatite (Zn-HA).
Methods
Zn-HA (2% and 6% Zn) was prepared and evaluated for the crystalline nature, chemical structure, and microstructure of hydroxyapatite (HA). Blank and 5-FU loaded alginate-gelatin-Zn-HA scaffolds were prepared and evaluated in terms of surface area, pore size, pore volume, and morphology using the gas adsorption technique and scanning electron microscopy (SEM). Cell attachment studies were also carried out on normal human cell line. The scaffolds were stored for one year at room temperature and evaluated for any changes in their physico-chemical properties as well as 5-FU release profile.
Results
The results showed that increasing zinc content decreased HA particle size. The blank scaffolds showed higher surface area, pore volume values, and a less porous structure compared to the drug-loaded ones. The pore size increased after scaffolds soaking in cell-line medium due to the hydrogel-forming materials' biodegradability and drug release. Cell growth on the presoaked drug-loaded scaffolds was improved compared to blank scaffold. XRD, FTIR and 5-FU release profile indicated that the stored scaffolds did not show any physical or chemical changes.
Conclusion
The prepared scaffolds showed promising results regarding cell attachment capability and long-term stability.
期刊介绍：
The Journal of Pharmaceutical Innovation (JPI), is an international, multidisciplinary peer-reviewed scientific journal dedicated to publishing high quality papers emphasizing innovative research and applied technologies within the pharmaceutical and biotechnology industries. JPI''s goal is to be the premier communication vehicle for the critical body of knowledge that is needed for scientific evolution and technical innovation, from R&D to market. Topics will fall under the following categories:
Materials science,
Product design,
Process design, optimization, automation and control,
Facilities; Information management,
Regulatory policy and strategy,
Supply chain developments ,
Education and professional development,
Journal of Pharmaceutical Innovation publishes four issues a year.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
